Initiation of teeth from the dental lamina in the ferret
Initiation of teeth from the dental lamina in the ferret
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DOI：
10.1016/j.diff.2013.11.004
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发表时间：
2014-01-01
期刊：
影响因子：
2.9
通讯作者：
Thesleff, Irma
中科院分区：
文献类型：
--
作者：
Jussila, Maria;Yanezl, Xenia Crespo;Thesleff, Irma
哺乳动物牙齿发育的特点是乳牙的形成，这些乳牙属于不同的牙齿类别，后来被单一的一组恒牙所取代。第一颗乳牙是由乳牙层开始的，替代牙是由乳牙舌侧的连续牙层开始的。牙间层将原牙细菌连接在一起。大多数哺乳动物的牙齿发育研究都是在老鼠身上进行的，老鼠没有所有牙齿类别的牙齿，没有替换牙齿，也没有发育牙间层。本文以雪貂（Mustela putorius furo）为模型动物，研究其牙间板发育和乳牙形成过程中的形态学变化和基因表达。此外，我们还分析了在牙齿置换过程中发生在牙间层和连续层的细胞-细胞信号传导。通过对连续组织切片的三维重建，我们观察到牙间板和乳牙的形态发生密切相关。Pitx2和Foxi3在牙间板表达表明其具有牙源性，并且在牙间板中有活跃的信号传导。Bmp4在其舌侧与干细胞因子Sox2共表达，这表明牙间层可能保留了形成牙齿的能力。我们发现，当牙齿替换开始时，出芽连续层和相邻间质中有Wnt通路活动，但没有活跃的Fgf或Eda信号。与人类牙齿替代表型相关的基因，包括Runx2和Il11r α，在雪貂中主要表达在连续层周围的间质中。我们的研究结果强调了牙板在哺乳动物牙齿发育过程中乳牙和替代牙的重要性。(c) 2013年国际分化学会。Elsevier B.V.版权所有。
Mammalian tooth development is characterized by formation of primary teeth that belong to different tooth classes and are later replaced by a single set of permanent teeth. The first primary teeth are initiated from the primary dental lamina, and the replacement teeth from the successional dental lamina at the lingual side of the primary teeth. An interdental lamina connects the primary tooth germs together. Most mammalian tooth development research is done on mouse, which does not have teeth in all tooth classes, does not replace its teeth, and does not develop an interdental lamina. We have used the ferret (Mustela putorius furo) as a model animal to elucidate the morphological changes and gene expression during the development of the interdental lamina and the initiation of primary teeth. In addition we have analyzed cell-cell signaling taking place in the interdental lamina as well as in the successional lamina during tooth replacement. By 3D reconstructions of serial histological sections we observed that the morphogenesis of the interdental lamina and the primary teeth are intimately linked. Expression of Pitx2 and Foxi3 in the interdental lamina indicates that it has odontogenic identity, and there is active signaling taking place in the interdental lamina. Bmp4 is coexpressed with the stem cell factor Sox2 at its lingual aspect suggesting that the interdental lamina may retain competence for tooth initiation. We show that when tooth replacement is initiated there is Wnt pathway activity in the budding successional lamina and adjacent mesenchyme but no active Fgf or Eda signaling. Genes associated with human tooth replacement phenotypes, including Runx2 and Il11r alpha, are mostly expressed in the mesenchyme around the successional lamina in the ferret. Our results highlight the importance of the dental lamina in the mammalian tooth development during the initiation of both primary and replacement teeth. (c) 2013 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.