Albumin improves stability and longevity of perfluorochemical-perfused hearts.
Albumin improves stability and longevity of perfluorochemical-perfused hearts.
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白蛋白可提高全氟化合物灌注心脏的稳定性和寿命。
DOI：
10.1152/ajpheart.1988.254.6.h1105
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Ensunsa,JL
中科院分区：
文献类型：
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作者：
Segel,LD;Ensunsa,JL
我们测定了蛋白质和非蛋白质促凝剂对全氟化学乳剂灌注的离体工作兔心脏的生理功能和亚结构完整性的影响。我们使用了四种培养基，其中含有Fluosol-43（FL）乳剂和3.4%羟乙基淀粉（HES）、2.9%白蛋白、0.8% HES或既不含HES也不含白蛋白（n = 5个心脏/组）。所有四组心脏在灌注的前5.5小时内功能稳定; FL加白蛋白心脏在大多数功能指标方面继续表现出稳定性，直到9.5小时。FL+白蛋白心脏的总射血功能时间（12.5 +/- 0.5 h）长于其他组（平均时间= 7.4-8.4 h）。功能稳定性和寿命与冠状动脉血流和冠状动脉血管阻力的维持相关。FL加白蛋白组的过量液体积聚和肌酸激酶渗漏率低于其他组。我们的结论是：1）白蛋白维持FL灌注心脏的功能、冠状动脉流量和心肌细胞完整性优于HES; 2）白蛋白可能通过维持毛细血管通透性发挥作用，从而降低间质液蓄积率并防止水肿诱导的血管压迫;和3）羟乙基淀粉对心脏功能或完整性没有影响，并且在FL中使用时不能有效防止间质液积聚。在缺乏蛋白质的情况下灌注离体心脏。
We determined the effect of protein and nonprotein oncotic agents on physiological function and substructural integrity of perfluorochemical emulsion-perfused isolated working rabbit hearts. We used four media that contained Fluosol-43 (FL) emulsion and either 3.4% hydroxyethylstarch (HES), 2.9% albumin, 0.8% HES, or neither HES nor albumin (n = 5 hearts/group). All four groups of hearts had stable function for the first 5.5 h of perfusion; the FL plus albumin hearts continued to exhibit stability in most indexes of function until 9.5 h. The FL plus albumin hearts had a longer total period of ejecting function (12.5 +/- 0.5 h) compared with the other groups (mean longevities = 7.4-8.4 h). Functional stability and longevity correlated with maintenance of coronary flow and coronary vascular resistance. The rates of excess fluid accumulation and creatine kinase leakage were lower in the FL plus albumin hearts than in the other groups. We conclude that: 1) albumin maintained function, coronary flow, and myocardial cell integrity of FL-perfused hearts better than did HES; 2) albumin may exert its effect by preserving capillary permeability, thereby reducing the rate of interstitial fluid accumulation and preventing edema-induced vascular compression; and 3) HES had no effect on cardiac function or integrity and was ineffective in preventing interstitial fluid accumulation when it was used in FL-perfused isolated hearts in the absence of protein.