On the hydration of DNA. II. Base composition dependence of the net hydration of DNA
On the hydration of DNA. II. Base composition dependence of the net hydration of DNA
复制标题
关于 DNA 的水合作用。
作者：
M. Tunis;J. Hearst
DNA的净水合作用对它的基本组成的依赖性已被测量的密度梯度超浓缩的三个DNA的在一系列的铯和锂盐溶液中的不同的水活度。外推到零水活度表明，部分特定体积对基础组合物的依赖性是非常小的CsDNA和空气为LiDNA。至少99%的浮力密度对基础组合物的依赖性，可以占的基础上的差分水合作用，与一摩尔的腺嘌呤-胸腺嘧啶对结合约2摩尔以上的水比一摩尔的鸟嘌呤-胞嘧啶对氯化铯。
The dependence of the net hydration of DNA on its base composition has been measured by density gradient ultracentrifugation of three DNA's in a series of cesium and lithium salt solutions of different water activities. Extrapolation to zero water activity showed the dependence of the partial specific volume on base composition to be very small for CsDNA and aero for LiDNA. At least 99% of the dependence of buoyant density on base composition can be accounted for on the basis of a differential hydration, with a mole of adenine–thymine pairs binding about 2 moles more water than a mole of guanine–cytosine pairs in CsCl.