Comprehensive MS and Solid-State NMR Metabolomic Profiling Reveals Molecular Variations in Native Periderms from Four Solanum tuberosum Potato Cultivars
Comprehensive MS and Solid-State NMR Metabolomic Profiling Reveals Molecular Variations in Native Periderms from Four Solanum tuberosum Potato Cultivars
复制标题
DOI：
10.1021/acs.jafc.6b05179
复制
发表时间：
2017-03-15
影响因子：
6.1
通讯作者：
Stark, Ruth E.
中科院分区：
文献类型：
--
作者：
Huang, Wenlin;Serra, Olga;Stark, Ruth E.
马铃薯（Solanum tuberosum L.）在世界粮食作物消费量中排名第三。尽管马铃薯皮的处理对食品工业构成了重大挑战，但这些组织中的次级代谢产物也具有生物活性，对作物发育至关重要。在整个块茎和伤口愈合组织中报道的不同的初级和次级代谢产物促使对来自四个具有独特皮肤形态和商业食品用途的品种的原生周皮进行全面的分析研究。同时提取极性和非极性可溶性代谢产物，进行色谱分析，并采用质谱法进行表征;通过固态C-13 NMR检查相应的固体界面聚合物残留物。总共有112个次生代谢产物中发现的叶部组织;多变量分析确定了10个极性和30个非极性的潜在生物标志物，区分一个单一的品种之间Norkotah赤褐色，大西洋，Chipeta，和育空地区黄金品种，具有对比russeting功能。成分的趋势被解释的背景下，围产期保护功能。
The potato (Solanum tuberosum L.) ranks third in worldwide consumption among food crops. Whereas disposal of potato peels poses significant challenges for the food industry, secondary metabolites in these tissues are also bioactive and essential to crop development. The diverse primary and secondary metabolites reported in whole tubers and wound-healing tissues prompted a comprehensive profiling study of native periderms from four cultivars with distinctive skin morphologies and commercial food uses. Polar and nonpolar soluble metabolites were extracted concurrently, analyzed chromatographically, and characterized with mass spectrometry; the corresponding solid interfacial polymeric residue was examined by solid-state C-13 NMR. In total, 112 secondary metabolites were found in the phellem tissues; multivariate analysis identified 10 polar and 30 nonpolar potential biomarkers that distinguish a single cultivar among Norkotah Russet, Atlantic, Chipeta, and Yukon Gold cultivars which have contrasting russeting features. Compositional trends are interpreted in the context of periderm protective function.