Oral cancer screening: serum Raman spectroscopic approach
Oral cancer screening: serum Raman spectroscopic approach
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DOI：
10.1117/1.jbo.20.11.115006
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发表时间：
2015-11-01
影响因子：
3.5
通讯作者：
Krishna, C. Murali
中科院分区：
文献类型：
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作者：
Sahu, Aditi K.;Dhoot, Suyash;Krishna, C. Murali
血清拉曼光谱（RS）先前在口腔癌诊断和复发预测中显示出潜力。为了评估血清RS在口腔癌筛查中的潜力，本研究对328名健康对照组、癌前病变组、疾病对照组和口腔癌组的受试者进行了癌前病变和癌症特异性检测。光谱用拉曼微探针获得。光谱结果表明，氨基酸、脂质、蛋白质、DNA和a-胡萝卜素在不同群体中的变化。采用主成分线性判别分析，以患者为中心进行数据分析。在第一步，在二元分类模型中评估癌前病变、疾病控制（非口腔癌）、口腔癌和正常样本的分类。此后，我们探索了两种筛选友好的分类方法，以进一步评估血清RS的临床应用：单一四组模型和正常与异常，然后确定异常模型的类型。结果证明了癌前病变和特异性肿瘤检测的可行性。正常与异常模型的敏感性和特异性分别为64%和80%；这些比率与标准筛查方法相当。由于目前目视检查的筛查方法主要适用于高危人群，因此血清RS可作为早期和特异性检测口腔癌前病变和癌症的有用辅助手段。（三）2015年中国光学仪器工程师学会
Serum Raman spectroscopy (RS) has previously shown potential in oral cancer diagnosis and recurrence prediction. To evaluate the potential of serum RS in oral cancer screening, premalignant and cancer-specific detection was explored in the present study using 328 subjects belonging to healthy controls, premalignant, disease controls, and oral cancer groups. Spectra were acquired using a Raman microprobe. Spectral findings suggest changes in amino acids, lipids, protein, DNA, and a-carotene across the groups. A patient-wise approach was employed for data analysis using principal component linear discriminant analysis. In the first step, the classification among premalignant, disease control (nonoral cancer), oral cancer, and normal samples was evaluated in binary classification models. Thereafter, two screening-friendly classification approaches were explored to further evaluate the clinical utility of serum RS: a single four-group model and normal versus abnormal followed by determining the type of abnormality model. Results demonstrate the feasibility of premalignant and specific cancer detection. The normal versus abnormal model yields better sensitivity and specificity rates of 64 and 80%; these rates are comparable to standard screening approaches. Prospectively, as the current screening procedure of visual inspection is useful mainly for high-risk populations, serum RS may serve as a useful adjunct for early and specific detection of oral precancers and cancer. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)