Premature centromere division of metaphase chromosomes in peripheral blood lymphocytes of Alzheimer's disease patients: relation to gender and age.
Premature centromere division of metaphase chromosomes in peripheral blood lymphocytes of Alzheimer's disease patients: relation to gender and age.
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DOI：
10.1093/gerona/glq148
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发表时间：
2010-12
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通讯作者：
L. Živković;B. Spremo-Potparević;B. Plećaš‐Solarović;N. Djelić;G. Ocić;P. Smiljković;S. Siedlak;Mark A. Smith;V. Bajic
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作者：
L. Živković;B. Spremo-Potparević;B. Plećaš‐Solarović;N. Djelić;G. Ocić;P. Smiljković;S. Siedlak;Mark A. Smith;V. Bajic
染色体改变是衰老和阿尔茨海默病（AD）的特征。这项研究检查了过早着丝粒分裂（PCD），AD中增加的染色体不稳定性指标，是否与衰老相关，或者相反，代表AD中加速衰老引起的从头染色体改变。在散发性AD患者和性别和年龄匹配的未受影响的对照组中测定外周血淋巴细胞中的PCD。分析中期细胞核中显示PCD的染色体、具有PCD的X染色体（PCD，X）和显示PCD的近端着丝粒染色体。AD患者，无论年龄大小，表现出增加的PCD上的任何染色体和PCD上的近端着丝粒染色体在两种性别，而增加的PCD的频率，X只在女性中表达。这种细胞遗传学分析表明，PCD是AD的一个特征，而不是时间老化的附带现象，并可能是有用的生理生物标志物，可用于疾病诊断。
Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.