Prenatal expression of the growth hormone (GH) receptor/binding protein in the rat: a role for GH in embryonic and fetal development?
Prenatal expression of the growth hormone (GH) receptor/binding protein in the rat: a role for GH in embryonic and fetal development?
复制标题
大鼠生长激素 (GH) 受体/结合蛋白的产前表达：GH 在胚胎和胎儿发育中的作用？
作者：
Juanita GARCIA;P. Lobie;G. Muscat;K. Gobius;G. Norstedt;M. Waters
虽然一般认为胎儿的生长不依赖于垂体生长激素(GH)，但垂体GH可能在器官发育中起着调节作用，或者非垂体来源的GH样物质可能通过GH受体影响胎儿的生长。因此，我们采用免疫组织化学、Northern印迹分析、逆转录聚合酶链式反应和溶液杂交等方法研究了12天龄大鼠胚胎(E12)到E18胎的生长激素受体/结合蛋白(BP)的个体发育。GH受体/BP免疫反应阳性反应见于E18胎鼠各主要器官系统，与胚层衍生物无明显联系。GH受体/BP免疫反应性在E12~E18有明显增加，其中E16~E18有显著增加。然而，血管母细胞组织和胎盘一样，在发育的所有阶段都有强烈或强烈的免疫反应。到第18天时，表达GH受体/BP免疫反应的其他组织中最值得注意的是骨骼肌和平滑肌、软骨前体细胞、上皮衬里细胞、神经神经节、室管膜细胞和肾上腺皮质。在胎盘中，最显著的免疫反应与蜕膜细胞有关。从E12~E18胎鼠和成年大鼠肝脏中提取总RNA。与35S标记的大鼠GH受体cRNA探针的Northern杂交显示，至少从E16开始，存在与大鼠GH受体核探针互补的3.9kb和1.2kb的转录本。聚合酶链式反应证实了胚胎12、14的生长激素受体基因的存在。
Although fetal growth is generally considered to be independent of pituitary growth hormone (GH), it is possible that pituitary GH plays a modulatory role in organ development or that a GH-like substance of non pituitary origin may influence fetal growth through the GH receptor. Accordingly, we have used immunohistochemistry, northern blot analysis, the reverse transcriptase-polymerase chain reaction and solution hybridization to study the ontogeny of the GH receptor/binding protein (BP) from the 12-day-old embryo (E12) to the E18 rat fetus. GH receptor/BP immunoreactivity was observed in all major organ systems of the E18 rat fetus and was not preferentially associated with any germ layer derivative. A general increase in GH receptor/BP immunoreactivity was evident from E12 to E18, with a marked increase occurring between E16 and E18. Hemangioblastic tissue was, however, strongly or intensely immunoreactive at all stages of development, as was the placenta. Most noteworthy of the other tissues expressing GH receptor/BP immunoreactivity by day 18 were skeletal and smooth muscle, chondroprogenitor cells, epithelial lining cells, neuronal ganglia, ependymal cells and the adrenal cortex. In the placenta, the most prominent immunoreactivity was associated with decidual cells. Total RNA was isolated from E12 to E18 rat fetuses and adult rat liver. Northern hybridization with a 35S-labelled rat GH receptor cRNA probe revealed that 3.9 kb and 1.2 kb transcripts complementary to the rat GH receptor riboprobe are present from at least E16. The existence of GH receptor mRNA at E12 and E14 was demonstrated by the polymerase chain reaction.(ABSTRACT TRUNCATED AT 250 WORDS)
人胎儿组织中的胎盘催乳素和生长激素受体：与胎儿血浆人胎盘催乳素浓度和胎儿生长的关系。
DOI：
10.1210/jcem-66-6-1283
发表时间：
1988
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Hill,DJ;Freemark,M;Strain,AJ;Handwerger,S;Milner,RD
通讯作者：
Milner,RD
DOI：
10.1073/pnas.86.20.8083
发表时间：
1989-10-01
影响因子：
11.1
作者：
GODOWSKI, PJ;LEUNG, DW;WOOD, WI
通讯作者：
WOOD, WI
肌肉发育中的胰岛素样生长因子（IGF）。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tollefsen,SE;Lajara,R;McCusker,RH;Clemmons,DR;Rotwein,P
通讯作者：
Rotwein,P