Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori
Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori
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DOI：
10.3748/wjg.15.3161
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发表时间：
2009-07-07
影响因子：
4.3
通讯作者：
Mohamed, Ramelah
中科院分区：
文献类型：
--
作者：
Ahmad, Norazah;Zakaria, Wan Rasinah;Mohamed, Ramelah
目的：研究克拉霉素耐药幽门螺杆菌(H Pylori)马来西亚分离株23S rRNA基因突变类型。用聚合酶链式反应扩增产物进行序列分析，分析克拉霉素耐药株和敏感株23S rRNA基因V区的点突变。用BSA I和MBO II酶对克拉霉素耐药株进行限制性内切酶片段长度多态性分析。结果：在120例患者的187株菌株中，4株对克拉霉素耐药，183株对克拉霉素敏感。耐药株的最低抑菌浓度为1.5~24微克/毫升。两个分离株有A2142G突变，另外两个有A2143G突变。在4株克拉霉素耐药株中有2株检出T2182C突变，在14株克拉霉素敏感株中检出13株突变。结论：克拉霉素耐药在Hp马来西亚分离株中并不常见，A2142G和A2143G突变与低水平耐药有关。(C)2009年《假发出版社》和《白石登》。版权所有。
AIM: To characterize the types of mutations present in the 23S rRNA genes of Malaysian isolates of clarithromycin-resistant Helicobacterpylori (H pylori).METHODS: Clarithromycin susceptibility of H pylori isolates was determined by E test. Analyses for point mutations in the domain V of 23S rRNA genes in clarithromycin-resistant and -sensitive strains were performed by sequence analysis of amplified polymerase chain reaction products. Restriction fragment length polymorphism was performed using Bsa I and Mbo II enzymes to detect restriction sites that correspond to the mutations in the clarithromycin-resistant strains.RESULTS: Of 187 isolates from 120 patients, four were resistant to clarithromycin, while 183 were sensitive. The MIC of the resistant strains ranged from 1.5 to 24 mu g/mL. Two isolates had an A2142G mutation and another two had A2143G mutations. A T2182C mutation was detected in two out of four clarithromycin-resistant isolates and in 13 of 14 clarithromycin-sensitive isolates. Restriction enzyme analyses with Bsa I and Mbo 11 were able to detect the mutations.CONCLUSION: Clarithromycin resistance is an uncommon occurrence among Malaysian isolates of H pylori strains and the mutations A2142G and A2143G detected were associated with low-level resistance. (C) 2009 The WIG Press and Baishideng. All rights reserved.