Use of the polycation polyethyleneimine to improve the physical properties of alginate-hyaluronic acid hydrogel during fabrication of tissue repair scaffolds
Use of the polycation polyethyleneimine to improve the physical properties of alginate-hyaluronic acid hydrogel during fabrication of tissue repair scaffolds
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DOI：
10.1080/09205063.2015.1016383
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发表时间：
2015-05-03
影响因子：
3.6
通讯作者：
Chen, Daniel X. B.
中科院分区：
文献类型：
--
作者：
Rajaram, Ajay;Schreyer, David J.;Chen, Daniel X. B.
最近，使用3D打印技术制造的基于藻酸盐的组织修复支架已被广泛研究用于组织工程应用。然而，它们的物理和机械性能对于许多组织工程应用是不利的，因为这些性能在制造过程中控制不良。通过添加透明质酸（HA）可以实现藻酸盐凝胶性质的一些改善，并且这也可以改善细胞与凝胶相互作用的能力。在这里，我们报告的藻酸盐-HA凝胶支架的物理性能的改善，通过在制造过程中加入聚阳离子聚乙烯亚胺（PEI），以稳定藻酸盐分子结构，通过形成一个稳定的复合物。我们发现PEI对藻酸盐-HA支架的物理性质具有显著的有益影响，包括凝胶溶胀程度的降低、支架降解速率的降低和凝胶的杨氏模量的增加。进一步的研究表明，用PEI制造藻酸盐-HA凝胶增加了牛血清白蛋白（一种模型蛋白）的包封效率，并降低了随后的初始蛋白释放速率。然而，还发现在支架制造过程中包封的施万细胞或ATDC-5软骨形成细胞的存活率随着PEI浓度的增加而适度降低。这项研究表明，在支架制造过程中使用PEI绘图可以提供一种有效的手段来控制藻酸盐为基础的支架性能的组织工程应用，但PEI的许多影响必须平衡在不同的情况下的最佳结果。
Recently alginate-based tissue repair scaffolds fabricated using 3D printing techniques have been extensively examined for use in tissue engineering applications. However, their physical and mechanical properties are unfavorable for many tissue engineering applications because these properties are poorly controlled during the fabrication process. Some improvement of alginate gel properties can be realized by addition of hyaluronic acid (HA), and this may also improve the ability of cells to interact with the gel. Here, we report improvement of the physical properties of alginate-HA gel scaffolds by the addition of the polycation polyethyleneimine (PEI) during the fabrication process in order to stabilize alginate molecular structure through the formation of a polyelectrolyte complex. We find that PEI has a significant beneficial influence on alginate-HA scaffold physical properties, including a reduction in the degree of gel swelling, a reduction in scaffold degradation rate, and an increase in the Young's modulus of the gel. Further study shows that fabrication of alginate-HA gels with PEI increases the encapsulation efficiency of bovine serum albumin, a model protein, and reduces the subsequent initial protein release rate. However, it was also found that survival of Schwann cells or ATDC-5 chondrogenic cells encapsulated during the scaffold fabrication process was modestly reduced with increasing PEI concentration. This study illustrates that the use of PEI during scaffold fabrication by plotting can provide an effective means to control alginate-based scaffold properties for tissue engineering applications, but that the many effects of PEI must be balanced for optimal outcomes in different situations.