Experimental approaches to investigating the vaginal biofilm microbiome.
Experimental approaches to investigating the vaginal biofilm microbiome.
复制标题
研究阴道生物膜微生物组的实验方法。
DOI：
10.1007/978-1-4939-0467-9_7
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Webster,Paul
中科院分区：
文献类型：
--
作者：
Baum,MarcM;Gunawardana,Manjula;Webster,Paul
Unraveling the complex ecology of the vaginal biofilm microbiome relies on a number of complementary techniques. Here, we describe the experimental approaches for studying vaginal microbial biofilm samples with a focus on specimen preparation for subsequent analysis. The techniques include fluorescence microscopy, fluorescence in situ hybridization, and scanning and transmission electron microscopy. Isolation of microbial DNA and RNA from these samples is covered along with a brief discussion of chemical analysis methods.
