Qualitative and quantitative analysis of phenolic compounds by UPLC-MS/MS and biological activities of Pholidota chinensis Lindl.
Qualitative and quantitative analysis of phenolic compounds by UPLC-MS/MS and biological activities of Pholidota chinensis Lindl.
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UPLC-MS/MS 定性定量分析石竹酚类化合物及其生物活性。
DOI：
10.1016/j.jpba.2020.113350
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发表时间：
2020-08-05
影响因子：
3.4
通讯作者：
Huang, Mingqing
中科院分区：
文献类型：
--
作者：
Ren, Mingcheng;Xu, Wen;Huang, Mingqing
石仙桃（Pholidota chinensis Lindl.）在中国常被用作功能茶或民间草药，但对其酚类成分及生物活性的研究较少。本研究旨在探究石仙桃经不同溶剂（石油醚、乙酸乙酯、正丁醇和水）提取所得提取物的酚类物质特征，以及它们的体外抗氧化、抗糖尿病和抗炎活性。结果表明，共准确鉴定出42种酚类化合物，其中30种通过超高效液相色谱 - 串联质谱（UPLC - MS/MS）在石仙桃提取物中得到进一步定量分析。在四种提取物中，乙酸乙酯提取物不仅总酚含量及21种单个酚类化合物含量最高，而且具有最强的抗氧化活性（DPPH法，1.43 mmol 抗坏血酸当量/克；ABTS法，5.38 mmol Trolox当量/克；FRAP法，2.58 mmol 硫酸亚铁铵当量/克）、α - 葡萄糖苷酶抑制活性（13.29 mmol 阿卡波糖当量/克）和抗炎活性（半数抑制浓度IC50 = 18.71 μg/ml）。此外，抗氧化、抗糖尿病和抗炎活性与总酚及单个酚类化合物显著相关。荭草苷和牡荆素可被视为石仙桃潜在的质量标志物。本研究将提供石仙桃全面的酚类物质特征，并将其与抗氧化、抗糖尿病和抗炎活性相关联。（C）2020爱思唯尔出版社。保留所有权利。
Pholidota chinensis Lindl. is commonly used as functional tea or folk medicine in China, but few studies have been conducted on its phenolic composition and bioactivities. This study was aimed to investigate the phenolic profiles of P. chinensis extracts by different solvents (petroleum ether, ethyl acetate, n-butanol and water) and their in vitro antioxidant, antidiabetic, and anti-inflammatory activities. The results showed that a total of 42 phenolic compounds were accurately identified, 30 of them were further quantified in P. chinensis extracts by UPLC-MS/MS. Among four extracts, ethyl acetate extract not only had the highest contents of total phenolics and 21 individual phenolic compounds, but also possessed the strongest antioxidant (DPPH, 1.43 mmol AAE/g; ABTS, 5.38 mmol TE/g; FRAP, 2.58 mmol FSHE/g), alpha-glucosidase inhibition (13.29 mmol ACAE/g) and anti-inflammation (IC50 = 18.71 mu g/ml) activities. Also, the antioxidant, antidiabetic, and anti-inflammatory activities were significantly correlated with total phenolics and individual phenolic compounds. Orientin and vitexin can be considered as the potential quality markers of P. chinensis. This study will provide a comprehensive phenolic profile of P. chinensis and relates them to their antioxidant, antidiabetic, and anti-inflammatory activities. (C) 2020 Elsevier B.V. All rights reserved.