A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment.
A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment.
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在动态环境中长期监测藻类的微流体平台。
DOI：
10.1021/acssynbio.5b00094
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发表时间：
2016-01-15
影响因子：
4.7
通讯作者：
Hasty J
中科院分区：
文献类型：
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作者：
Luke CS;Selimkhanov J;Baumgart L;Cohen SE;Golden SS;Cookson NA;Hasty J
在微流控“芯片实验室”中培养细胞用于延时显微镜已经成为研究生物系统动力学的有价值的工具。尽管微流体技术已被应用于培养和监测各种细菌和真核生物物种，但蓝细菌和真核微藻存在几个挑战，使得它们难以在微流体环境中培养。在这里，我们提出了一个可定制的设备，用于长期培养和成像的三个良好的特点菌株的蓝藻和微藻。这个平台有几个优势，琼脂糖垫，并展示了巨大的潜力，获得高品质，单细胞的蓝藻和藻类的基因表达数据在精确控制，动态环境中，在很长一段时间。
Culturing cells in microfluidic “lab-on-a-chip” devices for time lapse microscopy has become a valuable tool for studying the dynamics of biological systems. Although microfluidic technology has been applied to culturing and monitoring a diverse range of bacterial and eukaryotic species, cyanobacteria and eukaryotic microalgae present several challenges that have made them difficult to culture in a microfluidic setting. Here, we present a customizable device for the long-term culturing and imaging of three well characterized strains of cyanobacteria and microalgae. This platform has several advantages over agarose pads and demonstrates great potential for obtaining high quality, single-cell gene expression data of cyanobacteria and algae in precisely controlled, dynamic environments over long time periods.