Facilitation of signal onset and termination by adenylyl cyclase.
Facilitation of signal onset and termination by adenylyl cyclase.
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腺苷酸环化酶促进信号的起始和终止。
DOI：
10.1126/science.283.5406.1328
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Patel,TB
中科院分区：
文献类型：
--
作者：
Scholich,K;Mullenix,JB;Wittpoth,C;Poppleton,HM;Pierre,SC;Lindorfer,MA;Garrison,JC;Patel,TB
刺激性异三聚体三磷酸鸟苷结合蛋白（G 蛋白）的 α 亚基 (Gsα) Gs 激活哺乳动物腺苷酸环化酶的所有亚型。腺苷酸环化酶（V 型）及其子结构域与 Gsα 相互作用，通过增加鸟苷三磷酸酶 (GTPase) 活性来促进 G 蛋白失活。腺苷酸环化酶及其子结构域还增强了异源三聚体 Gs 的受体介导的激活，从而促进信号传导的快速启动。这些发现表明，腺苷酸环化酶作为单体 Gsα 的 GTP 酶激活蛋白 (GAP) 发挥作用，并增强受体的 GTP/GDP 交换因子 (GEF) 活性。
The α subunit (Gsα) of the stimulatory heterotrimeric guanosine triphosphate binding protein (G protein) Gsactivates all isoforms of mammalian adenylyl cyclase. Adenylyl cyclase (Type V) and its subdomains, which interact with Gsα, promoted inactivation of the G protein by increasing its guanosine triphosphatase (GTPase) activity. Adenylyl cyclase and its subdomains also augmented the receptor-mediated activation of heterotrimeric Gsand thereby facilitated the rapid onset of signaling. These findings demonstrate that adenylyl cyclase functions as a GTPase activating protein (GAP) for the monomeric Gsαand enhances the GTP/GDP exchange factor (GEF) activity of receptors.