Shape-transformation of polymersomes from glassy and crosslinkable ABA triblock copolymers
Shape-transformation of polymersomes from glassy and crosslinkable ABA triblock copolymers
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DOI：
10.1039/d0tb01643h
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发表时间：
2020-10-14
影响因子：
7
通讯作者：
Simon, Yoan C.
中科院分区：
文献类型：
--
作者：
Chidanguro, Tamuka;Ghimire, Elina;Simon, Yoan C.
聚合物囊泡（即聚合物囊泡）领域的最新进展已经证明，破坏环境的平衡条件可以导致形状转变为稳定的非球形形态，从而实现按需形状控制，并为细胞模拟和各种生物医学应用带来巨大希望。本文研究了玻璃态两亲性三嵌段共聚物聚乙二醇-嵌段-聚苯乙烯-静态-聚（香豆素甲基丙烯酸酯）-嵌段-聚乙二醇（PEG-b-P（S-stat-CMA）-b-PEG）的自组装行为及其对各种刺激的响应。通过改变疏水性P（S-stat-CMA）和亲水性PEG嵌段各自的分子量，我们改变了疏水性体积分数，从而从球形和蠕虫状胶束以及聚合物囊泡获得一系列形态。对于后者，我们观察到，缓慢的渗透压变化引起的透析导致的尺寸减小，而快速的渗透压变化通过添加PEG融合剂导致形态转化成棒状和管状聚合物囊泡。我们还发现，在诱导渗透压变化之前化学交联囊泡导致囊泡表现出低渗休克，这对于玻璃状聚合物囊泡是非典型的。我们相信，这种方法结合了三嵌段共聚物和基于光的转换的鲁棒性，将有助于扩大工具箱，设计更复杂的仿生结构。
Recent developments in the field of polymer vesicles,i.e.polymersomes, have demonstrated that disrupting the equilibrium conditions of the milieu could lead to shape transformation into stable non-spherical morphologies, bringing on-demand shape control to reality and bearing great promise for cell mimicry and a variety of biomedical applications. Here, we studied the self-assembly behavior of glassy amphiphilic triblock copolymers, poly(ethylene glycol)-block-polystyrene-stat-poly(coumarin methacrylate)-block-poly(ethylene glycol) (PEG-b-P(S-stat-CMA)-b-PEG), and their response to various stimuli. By changing the respective molecular weights of both the hydrophobic P(S-stat-CMA) and the hydrophilic PEG blocks, we varied the hydrophobic volume fraction thereby accessing a range of morphologies from spherical and worm-like micelles, as well as polymersomes. For the latter, we observed that slow osmotic pressure changes induced by dialysis led to a decrease in size while rapid osmotic pressure changes by addition of a PEG fusogen led to morphological transformations into rod-like and tubular polymersomes. We also found out that chemically crosslinking the vesicles before inducing osmotic pressure changes led to the vesicles exhibiting hypotonic shock, atypical for glassy polymersomes. We believe that this approach combining the robustness of triblock copolymers and light-based transformations will help expand the toolbox to design ever more complex biomimetic constructs.