Integrated label-free protein detection and separation in real time using confined surface plasmon resonance imaging
Integrated label-free protein detection and separation in real time using confined surface plasmon resonance imaging
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DOI：
10.1021/ac061932
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发表时间：
2007-03-15
影响因子：
7.4
通讯作者：
Tao, Nongjian
中科院分区：
文献类型：
--
作者：
Ly, Nguyen;Foley, Kyle;Tao, Nongjian
我们展示了一种无标记的蛋白质检测和分离技术，用于实时监测微/纳米流体通道中的蛋白质，密闭表面等离子体共振成像（restricted - spri）。这是通过在专门的SPRi传感器表面上直接制造超薄流体通道（500纳米高，500 μ m宽）来实现的。通过这种方式，SPRi独特地用于检测深入流体通道的蛋白质，同时保持分离产物的高横向精度。通道流体和蛋白质在外电场下被电动驱动。为了实现这一点，金属SPR传感器（46纳米的Au和2纳米的Cr）被分割成正方形阵列（每个正方形大小为200 μ m × 200 μ m，间隔8 μ m），并涂上30 nm的CYTOP聚合物。在这项工作中，我们通过一个简单的交叉结流体装置，在30 V/cm电场强度下，通过8毫米的分离通道，实时跟踪无标记蛋白质的分离。
We demonstrate a label-free protein detection and separation technology for real-time monitoring of proteins in micro/nanofluidic channels, confined surface plasmon resonance imaging (confined-SPRi). This was achieved by fabricating ultrathin fluidic channels (500 nm high, 500 mu m wide) directly on top of a specialized SPRi sensor surface. In this way, SPRi is uniquely used to detect proteins deep into the fluidic channel while maintaining high lateral accuracy of separated products. The channel fluid and proteins were driven electrokinetically under an external electric field. For this to occur, the metallic SPR sensor (46 nm of Au on 2 nm of Cr) was segmented into an array of squares (each 200 mu m x 200 mu m in size and spaced 8 mu m apart) and coated with 30 nm of CYTOP polymer. In this work, we track label-free protein separation in real time through a simple cross-junction fluidic device with an 8-mm separation channel length under 30 V/cm electric field strength.