The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis.
The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis.
复制标题
新型转录因子 HP1BP3 负调控香港牡蛎 Hsp70 转录
DOI：
10.1038/s41598-017-01573-y
复制
发表时间：
2017-05-03
影响因子：
4.6
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Xu D;Yang Q;Cui M;Zhang Q
CHHP1BP3是一种具有动态特征的染色质复合物相关蛋白，通过DNA亲和力纯化和质谱分析，将其鉴定为Crassostrea Hongkongensis中与CHHSP70启动子相关因子。使用EMSA证明了纯化的CHHP1BP3与CHHSP70启动子区域之间的直接相互作用。 CHHP1BP3耗竭导致香港梭菌血细胞中CHHSP70 mRNA表达的明显增强。但是，异源HEK293T细胞中的CHHP1BP3过表达与CHHSP70转录中的波动相关。定量RT-PCR分析表明，CHHSP70和CHHP1BP3转录都对热，CDCL2和NP对外部物理/化学应力有反应。这表明在压力诱导的遗传调节途径中，CHHSP70和CHHP1BP3之间存在合理的相关性。而物理和化学应力上的独特CHHP1BP3表达模式表明，介导CHHP1BP3诱导的机制可能是应力特异性的。这项研究发现了HP1BP3作为控制Hongkongensis中HSP70转录的负调节器的新作用，并有助于更好地理解有关HSP70转录的复杂调节机制。
ChHP1BP3, a chromatin complex-related protein known with dynamic features, was identified as a ChHsp70 promoter-associated factor in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. Direct interaction between purified ChHP1BP3 and the ChHsp70 promoter region was demonstrated using EMSA. ChHp1bp3 depletion led to clear enhancements in ChHsp70 mRNA expression in C. hongkongensis hemocytes. However, ChHp1bp3 overexpression in heterologous HEK293T cells correlated with fluctuations in ChHsp70 transcription. Quantitative RT-PCR analysis showed that both ChHsp70 and ChHp1bp3 transcription were responsive to external physical/chemical stresses by heat, CdCl2 and NP. This indicated a plausible correlation between ChHsp70 and ChHp1bp3 in the stress-induced genetic regulatory pathway. While, the distinctive ChHp1bp3 expression patterns upon physical and chemical stresses suggest that the mechanisms that mediate ChHp1bp3 induction might be stress-specific. This study discovered a novel role for HP1BP3 as a negative regulator in controlling Hsp70 transcription in C. hongkongensis, and contributed to better understanding the complex regulatory mechanisms governing Hsp70 transcription.