The NB protein is an integral component of the membrane of influenza B virus
The NB protein is an integral component of the membrane of influenza B virus
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DOI：
10.1099/0022-1317-77-11-2689
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发表时间：
1996-11-01
影响因子：
3.8
通讯作者：
Hay, AJ
中科院分区：
文献类型：
--
作者：
Betakova, T;Nermut, MV;Hay, AJ
生化和免疫电子显微镜的研究结果表明，Nb蛋白是B型流感病毒粒子的组成部分，其糖基化和膜定位与病毒感染细胞质膜上的Nb蛋白相同。对蛋白酶K的敏感性表明，N端位于病毒粒子的外部，而冻裂复制品的金免疫标记表明，C端位于病毒粒子的内部，B型流感病毒的Nb与甲型流感病毒M2的结构特征相似，它们存在于病毒粒子中，并具有离子通道活性，提示与M2蛋白类似，Nb也可能在病毒进入过程中发挥作用。
The results of biochemical and immunoelectron microscopic studies provide evidence that the NB protein is an integral component of the influenza B virion, Its glycosylation and orientation in the membrane were shown to be equivalent to that of NB in the plasma membrane of virus-infected cells. Sensitivity to proteinase K showed that the N terminus is exterior to the virion and gold immunolabelling of freeze-fractured replicas showed that the C terminus is located in the interior of the virion, The similarities between NB of influenza B and M2 of influenza A viruses in structural features, their presence in the virion and possession of an ion channel activity suggest that, by analogy with the M2 protein, NB may also have a role in virus entry.