Maternal low-protein diet suppresses vascular and renal endothelial NO synthase phosphorylation in rat offspring independent of a postnatal fructose diet
Maternal low-protein diet suppresses vascular and renal endothelial NO synthase phosphorylation in rat offspring independent of a postnatal fructose diet
复制标题
母体低蛋白饮食抑制大鼠后代血管和肾内皮 NO 合酶磷酸化，与产后果糖饮食无关
DOI：
10.1017/s2040174411000110
复制
发表时间：
2011
影响因子：
1.7
通讯作者：
Norikura T
中科院分区：
文献类型：
--
作者：
Sato S;Mukai Y;Norikura T
我们研究了出生后果糖饮食对暴露于蛋白质限制的母鼠后代的程序性高血压和血管及肾功能障碍的影响。妊娠Wistar大鼠在妊娠期和哺乳期分别饲喂对照组和低蛋白饲料。从哺乳期结束时起，雄性后代接受标准食物或60%果糖饮食：妊娠期和哺乳期的对照饮食和哺乳期结束时的对照饮食、对照组-对照组（CC）、60%果糖饮食-对照组（CF）、对照组-低蛋白饮食（LPC）和60%果糖饮食-低蛋白饮食（LPF）。治疗期间测量收缩压（SBP）。于生后94-101天，检测大鼠24 h尿中硝酸盐/亚硝酸盐（NOx）含量、主动脉和肾脏内皮型一氧化氮合酶（eNOS）蛋白水平、内皮素-1（ET-1）mRNA水平和NAD（P）H氧化酶亚单位。生后第97-101天收缩压（SBP），CF组（137 ± 2 mmHg，P < 0.05），LPC组（135 ± 1 mmHg，P < 0.05），LPF组（141 ± 2 mmHg，P < 0.05）均高于CC组（124 ± 1 mmHg，P < 0.05）。与CC组相比，CF组、LPC组和LPF组大鼠主动脉和肾组织尿NOx含量和eNOS磷酸化水平均降低。在主动脉中，LPC中NAD（P）H氧化酶亚基p47 phox和LPC、LPF中ET-1的mRNA水平均升高。这些结果表明，母体蛋白质限制升高血压，下调一氧化氮的产生和eNOS磷酸化，而出生后的果糖饮食没有显着差异，这些变化。
We investigated the effects of a postnatal fructose diet on the programmed hypertension and vascular and renal dysfunction in offspring from dams exposed to protein restriction. Pregnant Wistar rats were fed control and low-protein diets during the gestation and suckling periods. From the end of lactation, male offspring received standard chow or a 60% fructose diet: a control diet in the gestation and suckling periods and a control diet from the end of lactation, control-on-control (CC), 60% fructose diet-on-control (CF), control-on-low-protein diet (LPC) and 60% fructose diet-on-low-protein diet (LPF). The systolic blood pressure (SBP) was measured during treatment. At postnatal days 94–101, urinary 24 h nitrate/nitrite (NOx) content, protein levels of endothelial nitric oxide synthase (eNOS) and mRNA levels of endothelin-1 (ET-1), and NAD(P)H oxidase subunits in the aorta and kidney were examined. The SBP at postnatal days 97–101 increased in CF (137 ± 2 mmHg, P < 0.05), LPC (135 ± 1 mmHg, P < 0.05) and LPF (141 ± 2 mmHg, P < 0.05), compared with CC (124 ± 1 mmHg). The urinary NOx contents and eNOS phosphorylation in the aorta and kidney of CF, LPC and LPF decreased when compared with CC. In the aorta, the mRNA levels of NAD(P)H oxidase subunits p47phox in LPC and ET-1 in LPC and LPF increased. These results indicate that maternal protein restriction elevated the blood pressure, the downregulated nitric oxide production and eNOS phosphorylation, whereas the postnatal fructose diet made no significant difference to these alterations.