Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells
Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells
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DOI：
10.1016/s0014-5793(01)02691-6
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发表时间：
2001-08-10
期刊：
影响因子：
3.5
通讯作者：
Wang, JJ
中科院分区：
文献类型：
--
作者：
Hu, CCA;Tang, CHA;Wang, JJ
研究证实，褐斑蛙核糖核酸酶（RC-RNase）和onconase具有抗肿瘤活性。虽然onconase诱导的细胞死亡的分子决定因素已经变得更加明确，但rc - rnase诱导的死亡途径目前仍然未知。在这里，我们证明了在caspase-3缺陷的MCF-7细胞中，rc - rnase诱导的分子级联反应不包括起始caspase-8和-9的激活。裂解时间表明，procaspase-2和-6可能在MCF-7细胞中被活性caspase-7加工。Caspase-7还负责poly （adp -核糖）聚合酶的裂解。此外，我们报道过表达Bcl-X-L可以提高RC-RNase和onconase处理的MCF-7细胞的存活率。(C) 2001年由Elsevier Science B.V.代表欧洲生化学会联合会出版。
Rana catesbeiana ribonuclease (RC-RNase) and onconase were proven to own anti-tumor activity. While molecular determinants of onconase-induced cell death have become more explicit, the RC-RNase-induced death pathway remains presently unknown. Here we demonstrated that RC-RNase-induced molecular cascades in caspase-3-deficient MCF-7 cells did not include activation of initiation caspase-8 and -9. Cleavage timing suggested that procaspase-2 and -6 might be processed by active caspase-7 in MCF-7 cells. Caspase-7 was also responsible for cleavage of the poly (ADP-ribose) polymerase. Furthermore, we reported that overexpression of Bcl-X-L could raise the survival rates of MCF-7 cells treated with RC-RNase and onconase. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.