DNA binding site for a factor(s) required to initiate simian virus 40 DNA replication.
DNA binding site for a factor(s) required to initiate simian virus 40 DNA replication.
复制标题
启动猿猴病毒 40 DNA 复制所需因子的 DNA 结合位点。
DOI：
10.1073/pnas.83.6.1646
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
DePamphilis,ML
中科院分区：
文献类型：
--
作者：
Yamaguchi,M;DePamphilis,ML
通过使用由J.J.Li和T.J.Kelly [（1984）Proc. Natl. Acad. sci. USA 81，6973 - 6977]。环状双链DNA质粒复制CV-1细胞的提取物中，只有当质粒含有猿猴病毒40 DNA复制（ori）的顺式作用的起始序列和提取物含有猿猴病毒40大肿瘤抗原。含有ori的质粒和在ori中或附近携带缺失的质粒之间的竞争用于鉴定结合启动DNA复制所需的限速因子的序列。最小结合位点（核苷酸72 - 5243）包括复制起始所需的猿猴病毒40 ori序列的一半（ori核心）加上在ori核心的晚期基因侧上的连续序列，其含有促进起始的富含G + C的重复（ori辅助）。该起始因子结合位点对猿猴病毒40 ori区域具有特异性，尽管它排除了高亲和力的大肿瘤抗原DNA结合位点。
Efficient initiation of DNA replication in the absence of nonspecific DNA repair synthesis was obtained by using a modification of the system developed by J.J. Li and T.J. Kelly [(1984) Proc. Natl. Acad. Sci. USA 81, 6973-6977]. Circular double-stranded DNA plasmids replicated in extracts of CV-1 cells only when the plasmids contained the cis-acting origin sequence for simian virus 40 DNA replication (ori) and the extract contained simian virus 40 large tumor antigen. Competition between plasmids containing ori and plasmids carrying deletions in and about ori served to identify a sequence that binds the rate-limiting factor(s) required to initiate DNA replication. The minimum binding site (nucleotides 72-5243) encompassed one-half of the simian virus 40 ori sequence that is required for initiation of replication (ori-core) plus the contiguous sequence on the late gene side of ori-core containing G + C-rich repeats that facilitates initiation (ori-auxiliary). This initiation factor binding site was specific for the simian virus 40 ori region, even though it excluded the high-affinity large tumor antigen DNA binding sites.
克隆的小鼠DNA片段可以在体内和体外的猿猴病毒40T抗原依赖性系统中复制
影响因子：
5.3
作者：
H. Ariga;Z. Tsuchihashi;M. Naruto;M. Yamada
通讯作者：
M. Yamada
来自猿猴细胞的 DNA 引发酶-DNA 聚合酶 α：猿猴病毒 40 DNA 上起始位点的序列特异性。
影响因子：
5.3
作者：
M. Yamaguchi;E. Hendrickson;M. DePamphilis
通讯作者：
M. DePamphilis