Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture.
Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture.
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DOI：
10.1186/2045-3701-4-40
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发表时间：
2014
影响因子：
7.5
通讯作者：
Tsai FJ
中科院分区：
文献类型：
--
作者：
Lin YJ;Chen CY;Jeang KT;Liu X;Wang JH;Hung CH;Tsang H;Lin TH;Liao CC;Huang SM;Lin CW;Ho MW;Chien WK;Chen JH;Ho TJ;Tsai FJ
人类免疫缺陷病毒（HIV-1）利用宿主蛋白质完成其生命周期。全基因组siRNA方法表明宿主蛋白影响HIV-1复制。然而，结果几乎没有重叠。RING Finger Protein 39（RNF 39）是一种在全基因组关联研究中发现的蛋白质。然而，其在HIV-1复制过程中的功能仍不清楚。我们研究了常见的RNF 39基因变异与HIV-1病毒载量之间的关系。然后在不同的细胞系中研究RNF 39蛋白敲低或过表达对HIV-1复制的影响。两种遗传变异与HIV-1病毒载量相关。具有ht 1-GG/GG单倍型的患者表现出较低的RNF 39表达水平和较低的HIV-1病毒载量。RNF 39敲低抑制HIV-1表达。RNF 39蛋白可能参与HIV-1复制，如在HIV-1患者的遗传学研究和体外细胞培养中观察到的。
The human immunodeficiency virus (HIV-1) exploits host proteins to complete its life cycle. Genome-wide siRNA approaches suggested that host proteins affect HIV-1 replication. However, the results barely overlapped. RING finger protein 39 (RNF39) has been identified from genome-wide association studies. However, its function during HIV-1 replication remains unclear. We investigated the relationship between common RNF39 genetic variants and HIV-1 viral loads. The effect of RNF39 protein knockdown or overexpression on HIV-1 replication was then investigated in different cell lines. Two genetic variants were associated with HIV-1 viral loads. Patients with the ht1-GG/GG haplotype presented lower RNF39 expression levels and lower HIV-1 viral load. RNF39 knockdown inhibited HIV-1 expression. RNF39 protein may be involved in HIV-1 replication as observed in genetic studies on patients with HIV-1 and in in vitro cell cultures.
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影响因子：
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影响因子：
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作者：
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