Effects of Fam83h overexpression on enamel and dentine formation.
Effects of Fam83h overexpression on enamel and dentine formation.
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DOI：
10.1016/j.archoralbio.2013.03.001
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发表时间：
2013-09
影响因子：
3
通讯作者：
Kim, Jung-Wook
中科院分区：
文献类型：
--
作者：
Kueon, Young-Sun;Lee, Kyung-Eun;Ko, Jiyeon;Hu, Jan C. -C.;Simmer, James P.;Kim, Jung-Wook
本研究的目的是确定FAM 83 H过表达是否导致牙本质或釉质畸形。将全长小鼠Fam 83 h cDNA插入到pCAGIG载体的β-肌动蛋白启动子和β-珠蛋白增强子之间，以在转基因小鼠中普遍表达。表达重组小鼠FAM 83 H并用于产生多克隆抗体。Western印迹显示Fam 83 h转基因的表达增强。通过牙釉质和牙本质显微硬度的测量来评估转基因表达对牙齿发育的影响。测量牙槽嵴水平的切牙釉质总厚度，并通过扫描电子显微镜（SEM）观察交叉杆图案。基于它们的转基因表达水平选择三个转基因小鼠系。转基因小鼠的牙釉质和牙本质的显微硬度值在转基因小鼠之间以及转基因小鼠与野生型小鼠之间均无显著性差异。在转基因系和野生型小鼠之间没有观察到釉质厚度的统计学显著差异。SEM分析显示，釉质晶体和棒形态没有明显差异。我们的研究结果表明，小鼠中FAM 83 H的过表达不会在牙本质或釉质中产生表型。
The aim of this study was to determine if FAM83H over-expression causes dentin or enamel malformations. The full-length mouse Fam83h cDNA was inserted into the pCAGIG vector between a β-actin promoter and β-globin enhancer for ubiquitous expression in transgenic mice. Recombinant mouse FAM83H was expressed and used to generate polyclonal antibodies. Western blots showed enhanced expression of the Fam83h transgene. The effects of transgene expression on tooth development were assessed by microhardness measurements of enamel and dentin. Total thickness of incisor enamel at the level of the alveolar crest was measured and decussating rod patterns were visualized by scanning electron microscopy (SEM). Three transgenic mouse lines were selected based upon their transgene expression levels. There was no statistically significant difference in the Vickers microhardness values of enamel or dentin between the transgenic lines or between the transgenic lines and wild type mice. No statistically significant differences in enamel thickness were observed between the transgenic lines and the wild type mice. SEM analysis revealed no apparent differences in the enamel crystal and rod morphologies. Our findings demonstrate that over-expression of FAM83H in mice does not produce a phenotype in dentin or enamel.
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影响因子：
4.5
作者：
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通讯作者：
Mellersh CS
影响因子：
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