A bio-nanocapsule containing envelope protein domain III of Japanese encephalitis virus protects mice against lethal Japanese encephalitis virus infection
A bio-nanocapsule containing envelope protein domain III of Japanese encephalitis virus protects mice against lethal Japanese encephalitis virus infection
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DOI：
10.1111/1348-0421.12055
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发表时间：
2013-06-01
影响因子：
2.6
通讯作者：
Arakawa, Takeshi
中科院分区：
文献类型：
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作者：
Miyata, Takeshi;Tafuku, Senji;Arakawa, Takeshi
将包含修饰的乙型肝炎B表面抗原L蛋白的工程化生物纳米胶囊（BNC）用作日本脑炎病毒（JEV）包膜蛋白结构域III（D3）的物理支架。在N末端，BNC含有来源于金黄色葡萄球菌蛋白A（ZZ-BNC）的Z结构域（ZZ）的双串联重复。暴露在ZZ-BNC表面的富含赖氨酸的ZZ部分用于与JEV D3抗原的化学缀合，该抗原已从大肠杆菌中表达和纯化。用负载在ZZ-BNC表面的D3（ZZ-BNC：D3）免疫小鼠增强了针对JEV的血清IgG应答，并增加了针对致死性JEV感染的保护。本研究表明，无害的重组抗原，当加载到ZZ-BNC的表面，可以转化为免疫原性抗原。
An engineered bio-nanocapsule (BNC) comprising modified hepatitis B surface antigen L protein was used as a physical scaffold for envelope protein domain III (D3) of Japanese encephalitis virus (JEV). At the N terminus, the BNC contained a two-tandem repeat of the Z domain (ZZ) derived from Staphylococcus aureus protein A (ZZ-BNC). The Lys-rich ZZ moiety exposed on the surface of ZZ-BNC was used for chemical conjugation with the JEV D3 antigen, which had been expressed and purified from Escherichia coli. Immunization of mice with D3 loaded on the surface of ZZ-BNC (ZZ-BNC:D3) augmented serum IgG response against JEV and increased protection against lethal JEV infection. The present study suggests that innocuous recombinant antigens, when loaded on the surface of ZZ-BNC, can be transformed to immunogenic antigens.