Voltage clamp of rat and human skeletal muscle: measurements with an improved loose‐patch technique.
Voltage clamp of rat and human skeletal muscle: measurements with an improved loose‐patch technique.
复制标题
大鼠和人类骨骼肌的电压钳：使用改进的松散贴片技术进行测量。
DOI：
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Ruff
中科院分区：
文献类型：
--
作者：
W. Almers;W. M. Roberts;R. Ruff
Intact fibres of human intercostal and rat omohyoid muscles were studied at 23 degree C with a loose‐patch voltage‐clamp technique that employed two concentric micropipettes to electrically isolate small‐diameter (10‐15 microns) patches of sarcolemma. This method allows investigation of membrane excitability under highly physiological conditions. Step depolarizations to 0 mV elicited sodium inward currents that reached peak values of up to 20 mA/cm2 within 250 microseconds, and then declined. In human muscle, the reversal potential (ENa) was approximately 40 mV, and maximal conductances (GNa) ranged from 44 to 360 mS/cm2. In rat muscle, ENa was 42 mV and GNa ranged from 100 to 250 mS/cm2. Sodium channels in rat and human muscle were indistinguishable in most aspects of their kinetic behaviour and voltage dependence. Outward potassium currents were small by comparison (usually less than 2 mA/cm2) and saturated at positive potentials. The maximum potassium conductance (GK) ranged from 0 to 19 mS/cm2 (human) and from 4 to 12 mS/cm2 (rat muscle).
通过玻璃微量移液器进行光漂白：青蛙骨骼肌肌膜中没有横向移动性的钠通道。
DOI：
10.1073/pnas.79.3.946
发表时间：
1982
影响因子：
11.1
作者：
Stühmer,W;Almers,W
通讯作者：
Almers,W
糖皮质激素引起的萎缩并不是由于大鼠肌肉的兴奋性受损所致。
DOI：
10.1152/ajpendo.1982.243.6.e512
发表时间：
1982
期刊：
The American journal of physiology
影响因子：
--
作者：
Ruff,RL;Martyn,D;Gordon,AM
通讯作者：
Gordon,AM