Rare earth elements detoxification mechanism in the hyperaccumulator Dicranopteris linearis: [silicon-pectin] matrix fixation.
Rare earth elements detoxification mechanism in the hyperaccumulator Dicranopteris linearis: [silicon-pectin] matrix fixation.
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DOI：
10.1016/j.jhazmat.2023.131254
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发表时间：
2023-03
影响因子：
13.6
通讯作者：
Hong-Xiang Zheng;Yu-Lu Yang;Wenshen Liu;Y. Zhong;Yue Cao;R. Qiu;Chong Liu;A. van der Ent;Martin John Hodson;Ye-tao Tang
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文献类型：
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作者：
Hong-Xiang Zheng;Yu-Lu Yang;Wenshen Liu;Y. Zhong;Yue Cao;R. Qiu;Chong Liu;A. van der Ent;Martin John Hodson;Ye-tao Tang
Dicranopteris线性是最著名的稀土元素（ree）和硅（Si）的超富集物种，能够处理稀土的毒性水平。因此，本研究旨在阐明d。线性叶片应对过多的稀土胁迫，以及Si是否在稀土解毒中起作用。结果表明，以镧（La -）为代表的稀土胁迫导致叶片生物量下降，与叶片细胞壁合成和修饰相关的代谢增加。然而，镧胁迫诱导的反应，特别是果胶相关基因表达水平、果胶多糖浓度和甲基酯酶活性的增加，可以通过硅的供应来缓解。大约70%的硅纳米线叶与细胞壁相互作用形成有机硅硅- o - c键。硅修饰的细胞壁含有更多的羟基，与不含硅的细胞壁相比，可以更有效地保留稀土元素。此外，该[硅细胞壁]基质使果胶- la积累能力提高了64%，而对半纤维素- la和纤维素- la积累能力没有影响。这些结果表明，[硅果胶]基质固定是稀土解毒的关键，为利用该物种在稀土污染场地开发植物技术应用（如稀土植物提取）奠定了基础。
Dicranopteris linearisis the best-known hyperaccumulator species of rare earth elements (REEs) and silicon (Si), capable of dealing with toxic level of REEs. Hence, this study aimed to clarify howD. linearisleaves cope with excessive REE stress, and whether Si plays a role in REE detoxification. The results show that lanthanum (La – as a representative of the REEs) stress led to decreased biomass and an increase of metabolism related to leaf cell wall synthesis and modification. However, the La stress-induced responses, especially the increase of pectin-related gene expression level, pectin polysaccharides concentration, and methylesterase activity, could be mitigated by Si supply. Approximately 70% of the Si inD. linearisleaves interacted with the cell walls to form organosilicon Si-O-C linkages. The Si-modified cell walls contained more hydroxyl groups, leading to a more efficient REE retention compared to the Si-free ones. Moreover, this [Si-cell wall] matrix increased the pectin-La accumulation capacity by 64%, with no effect on hemicellulose-La and cellulose-La accumulation capacity. These results suggest that [Si-pectin] matrix fixation is key in REE detoxification inD. linearis, laying the foundation for the development of phytotechnological applications (e.g., REE phytomining) using this species in REE-contaminated sites.