Differential effects of inhibition of polyamine biosynthesis on cell cycle traverse and structure of the prematurely condensed chromosomes of normal and transformed cells
Differential effects of inhibition of polyamine biosynthesis on cell cycle traverse and structure of the prematurely condensed chromosomes of normal and transformed cells
复制标题
抑制多胺生物合成对正常细胞和转化细胞的细胞周期遍历和过早浓缩染色体结构的不同影响
DOI：
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发表时间：
1979
影响因子：
5.6
通讯作者：
P. Rao
中科院分区：
文献类型：
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作者：
P. Sunkara;Mary B. Pargac;K. Nishioka;P. Rao
本研究的目的是确定细胞周期中正常细胞和转化细胞因多胺剥夺而停滞的点。用甲基乙二醛双（脒基腙）处理正常细胞（人成纤维细胞系 PA2 和小鼠 3T3）和转化细胞（CHO、HeLa 和 SV3T3）会导致亚精胺和精胺水平显着降低，这与生长抑制有关。对多胺耗尽细胞的过早浓缩染色体（PCC）的检查表明，正常成纤维细胞优先停滞在早期 G1 期，而转化系中的大多数细胞则停滞在 S 期。对转化细胞 PCC 的仔细检查表明 DNA 复制位点数量显着减少，表明多胺在 DNA 链起始中具有重要作用。
The objective of this study was to determine the points in the cell cycle at which normal and transformed cells become arrested as a result of polyamine deprivation. Treatment of normal (human fibroblast line PA2 and mouse 3T3) and transformed (CHO, HeLa and SV3T3) cells with methylglyoxal bis‐(guanyl‐hydrazone) resulted in a significant decrease in the levels of spermidine and spermine which was associated with an inhibition of growth. Examination of the prematurely condensed chromosomes (PCC) of the polyaminedepleted cells, revealed that normal fibroblasts were preferentially arrested in early G1 phase while a majority of cells in the transformed lines were blocked in S phase. A close examination of the PCC of the transformed cells indicated a significant decrease in the number of DNA replication sites suggesting that polyamines have an important role in DNA chain initiation.