Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE.
Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE.
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DOI：
10.1016/s1001-0742(08)62158-0
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发表时间：
2008
影响因子：
6.9
通讯作者：
Yanling Ying;Z. Lv;H. Min;Jun Cheng
中科院分区：
文献类型：
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作者：
Yanling Ying;Z. Lv;H. Min;Jun Cheng
采用聚合酶链反应变性梯度凝胶电泳（DGGE）技术，对光合产氢过程中微生物种群的动态变化进行了监测。直接从反应器中的混合菌群中提取总DNA，用V3- 16 SrDNA和pufM基因引物进行PCR扩增，并对扩增产物进行DGGE分析。DGGE图谱反映了反应器不同运行阶段的群落结构动态和微生物多样性变化。最佳产氢群落在第10天形成。利用pufM基因片段进行DGGE分析比利用V3- 16 SrDNA区域基因片段进行DGGE分析更能反映光合细菌产氢过程中的种群动态变化。DGGE条带的比较序列分析显示了特定群体结构与系统性能之间的关系。沼泽红球藻被认为是反应器中产氢的优势群落成员之一。PCR-DGGE技术是一种真实的实时监测光产氢过程的有效工具，为光产氢系统的改进提供了有效的信息。
A PCR-DGGE (denaturing gradient gel electrophoresis of polymerase chain reaction) protocol was used for monitoring the dynamic changes in the microbial population during photohydrogen production. Total DNA was extracted directly from the mixed bacterial community in the reactor and subjected to PCR with V3–16S rDNA and pufM gene primers, and the amplifications were then analyzed by DGGE. The DGGE patterns demonstrated the dynamics of community structure and the shift of microbial diversity, which corresponded to different running periods of the reactor. The optimal hydrogen producing community formed on day 10. Using DGGE analysis with the pufM gene fragments was superior to V3–16S rDNA region genes for detecting the dynamic variations of the photosynthetic bacteria population during hydrogen production. The comparative sequence analysis of excised DGGE bands showed the relationship between specific population structures and system performance. Rhodopseudomonas palustris was presumed as one of the dominant community members for hydrogen production in the reactor. The PCR-DGGE protocol was proven to be a good tool for monitoring the photohydrogen production in real time and offered the available information to improve the photohydrogen producing system.