Transcriptional analysis of disk abalone (Haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge
Transcriptional analysis of disk abalone (Haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge
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DOI：
10.1016/j.fsi.2011.04.001
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发表时间：
2011-07-01
影响因子：
4.7
通讯作者：
Lee, Jehee
中科院分区：
文献类型：
--
作者：
De Zoysa, Mahanama;Whang, Ilson;Lee, Jehee
多种抗氧化酶对于海洋生物克服氧化应激以及通过激活不同的信号转导途径来微调免疫反应是必不可少的。本研究采用细菌激发法对盘鲍抗氧化酶基因进行了转录分析在细菌和VHSV攻击后，锰超氧化物歧化酶（MnSOD）、铜锌超氧化物歧化酶（CuZnSOD）、过氧化氢酶、硫氧还蛋白过氧化物酶（TPx）、硒依赖性谷胱甘肽过氧化物酶（SeGPx）、和硫氧还蛋白-2（TRx-2）的表达水平在鳃和血细胞中以不同程度改变。在鳃，只有MnSOD，过氧化氢酶，SeGPx基因完全上调后的挑战的细菌和VHSV。其中，SeGPx在鳃中表现出16倍（细菌）和2倍（VHSV）的强烈上调，在血细胞中表现出5倍（细菌）和3.0倍（VHSV）的强烈上调。没有一个基因被下调（鳃和血细胞）细菌的挑战，即使CuZnSOD和TPx显示下调（完全）VHSV血细胞。总体而言，鲍鱼血细胞在细菌和VHSV攻击时诱导抗氧化酶转录的潜力低于鳃。基于这些结果，我们认为鲍鱼在细菌和VHSV攻击期间诱导组织中的氧化应激，抗氧化酶的响应可以支持维持低水平的活性氧（ROS）其可以作为激活针对致病性条件的免疫反应的信号。(C)2011爱思唯尔有限公司保留所有权利。
Diverse antioxidant enzymes are essential for marine organisms to overcome oxidative stress as well as for the fine-tuning of immune reactions through activating different signal transduction pathways. This study describes the transcriptional analysis of antioxidant enzymes of disk abalone by challenging with bacteria (Vibrio alginolyticus, Vibrio parahemolyticus, and Listeria monocytogenes) and viral hemorrhagic septicemia virus (VHSV).Upon bacteria and VHSV challenge, Manganese superoxide dismutase (MnSOD), Copper, Zinc superoxide dismutase (CuZnSOD), catalase, thioredoxin peroxidase (TPx), Selenium-dependent glutathione peroxidase (SeGPx), and thioredoxin-2 (TRx-2) expression levels were altered in gills, and hemocytes at different magnitudes. In gills, only MnSOD, catalase, and SeGPx genes were completely upregulated by post-challenge of bacterial and VHSV. Among them, SeGPx demonstrated strong upregulation by 16-fold (bacteria) and 2-fold (VHSV) in gills, and 5-fold (bacteria) and 3.0-fold (VHSV) in hemocytes. None of the genes examined were downregulated (in gills and hemocytes) by bacteria challenge even though CuZnSOD and TPx showed downregulation (completely) in hemocytes by VHSV. In general, abalone hemocytes had lower potential to induce antioxidant enzyme transcripts upon bacteria and VHSV challenge than gills.Based upon these results, we suggest that abalones induce oxidative stress in tissues during the bacteria and VHSV challenge, and the identified response of antioxidant enzymes could be supported for maintaining a low-level of reactive oxygen species (ROS) that may serve as a signal for activating immune reactions against pathogenic conditions. (C) 2011 Elsevier Ltd. All rights reserved.