PHBHHx facilitated the residence, survival and sternness maintain of transplanted neural stem cells in traumatic brain injury rats
PHBHHx facilitated the residence, survival and sternness maintain of transplanted neural stem cells in traumatic brain injury rats
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PHBHHx促进脑外伤大鼠移植神经干细胞的滞留、存活和稳定性维持
DOI：
10.1021/acs.biomac.9b00408
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发表时间：
2019
影响因子：
6.2
通讯作者：
Lu Xiaoyun
中科院分区：
文献类型：
--
作者：
Wang Leilei;Yang Zhiqian;Fang Fan;Sun Shuhong;Wu Xingjuan;Lu Haixia;Lu Xiaoyun
Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a polyhydroxyalkanoate (PHA) with broad application prospects in various biomedical fields. Our previous study demonstrated the great biocompatibility of PHBHHx with neural stem cells (NSCs)in vitro. In this study, we went a step further and implanted the NSC-carrying PHBHHx film toin vivointo the lesion region in a rat traumatic brain injury (TBI) model. Thein vivobiocompatibility of PHBHHx, as well as the survival, migration and differentiation of the transplanted NSCs were investigated. The results showed that PHBHHx did not induce additional reactive gliosis and supported thein vivosurvival and growth of the transplanted stem cells. The transplanted NSCs were able to migrate into the brain tissue and differentiated into both neurons and astrocytes. Moreover, PHBHHx seemed to be able to maintain the stemness of the attached NSCs bothin vitroandin vivo. The major monomer of PHBHHx, 3-hydroxybutyrate (3-HB), might contribute to this bioactivity because the addition of low concentrations of 3-HB facilitated the self-renewal of NSCs by shortening the G1 phase, promoted their proliferation, and enhanced the expression of a key regulatory transcription factor which helped to keep the stemness of NSCs. These results highlight the application of PHBHHx as a promising bioactive material for NSC transplantation in the brain and open up a wide space for further studies on functional recovery following the transplantation of NSCs attached to PHBHHx.
