Genetic diversity analysis of hulless barley from Shangri-la region revealed by SSR and AFLP markers
Genetic diversity analysis of hulless barley from Shangri-la region revealed by SSR and AFLP markers
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DOI：
10.1007/s10722-011-9783-5
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发表时间：
2012-10-01
影响因子：
2
通讯作者：
Long, Chunlin
中科院分区：
文献类型：
--
作者：
Guo, Yu;Li, Yali;Long, Chunlin
为将香格里拉地区的青稞资源加入全球青稞资源库，本研究对该地区青稞资源的遗传多样性进行了初步的研究。利用SSR和AFLP标记对云南香格里拉地区3个县60份青稞样品的遗传多样性进行了研究。19对SSR引物共检测到70个等位基因，5对AFLP引物共扩增出525条带，其中多态性条带464条。SSR引物的多态性信息含量（PIC）在0.03 ~ 0.86之间。3个群体的等位基因总数分别为51、55、43，多态性带分别为188、205、141。3个群体间的遗传距离和遗传一致性表明它们之间的亲缘关系较近。群体间的基因多样性相对于总的群体多样性（Gst）为0.13 SSR标记和0.02 AFLP标记，表明只有13%和2%的变异，分别在人群中。UPGMA聚类分析结果表明，所有的样本都是随机分组，而不是根据其种群、行型和春秋季类型而聚为不同的类群。结果表明，香格里拉地区种群具有较高的遗传多样性，其遗传多样性的形成与复杂的生态环境和当地居民的传统习俗有关。
For adding the hulless barley resources of Shangri-la region to the global barley resource library, a basic work was done by us to assess their genetic diversity of this region. The genetic diversity of 60 hulless barley samples collected from three counties in Shangri-la region of Yunnan Province, were studied using SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphism) markers. A total of 70 alleles were detected for 19 pairs of SSR primers, and 525 band containing 464 polymorphic bands were revealed for 5 pairs of AFLP primers. The value of polymorphism information content (PIC) ranged from 0.03 to 0.86 for SSR primers. The total numbers of alleles were 51, 55, 43 in three populations and the polymorphic bands were 188, 205 and 141. The genetic distances and genetic identity among the three populations showed their close relationship. The gene diversity among populations relative to the total population diversity (Gst) was 0.13 for SSR markers and 0.02 for AFLP markers and indicated that just 13 and 2% variations were among populations, respectively. The UPGMA cluster analysis revealed that all of the samples grouped randomly rather than clustered into distinct groups corresponding to their populations, row types and spring/fall types. We concluded that there was high genetic diversity in the population of Shangri-la region and the formation of diversity was related to complex environment and inhabitants' traditional practices.