How to convincingly measure low concentration samples with optical label-free biosensors
How to convincingly measure low concentration samples with optical label-free biosensors
复制标题
如何使用光学无标记生物传感器令人信服地测量低浓度样品
DOI：
10.1016/j.snb.2019.127568
复制
发表时间：
2020
影响因子：
8.4
通讯作者：
Sun Xiaqing
中科院分区：
文献类型：
--
作者：
Yang Tian;Chen Shengfu;He Xiaolong;Guo Huaizu;Sun Xiaqing
在过去的二十年里，在光学无标记生物传感器件的科学研究方面取得了巨大的进展，包括等离子体和光学谐振器，波导和元结构。现在是时候将先进的设备技术与真实的世界的生物传感应用相结合，并找到工业突破。在这一点上，对于器件研究界来说，就这些器件的真正功能和性能限制以及实现所需应用的关键技术挑战形成正确和共同的观点是非常重要的。在本文中，我们专注于指导如何令人信服地测量低浓度的生物分子样品，并支持检测限（LOD）的要求。我们将首先评论这个研究领域的核心价值和陷阱，并简要回顾基本物理原理。然后，在详细阐述了生物分子相互作用过程和干扰因素后，我们提供了一份清单，列出了测量低浓度生物分子样品和支持LOD声明的要点和正确做法。
Over the past two decades, tremendous progress has been made in scientific research on optical label-free biosensing devices, including plasmonic and optical resonators, waveguides and meta-structures. Now it is time to combine the advanced device technologies with real world biosensing applications and find industrial breakthroughs. At this point, it is very important for the device research community to form a correct and common view about the true capabilities and performance limits of these devices, and about the key technical challenges in our way to the desired applications. In this paper, we focus on instructing how to measure low concentration biomolecule samples convincingly and support limit-of-detection (LOD) claims. We will first comment on the core values and pitfalls of this research area, and briefly review the fundamental physical principles. Then after elaborating on the biomolecule interaction process and the interference factors, we provide a checklist of the essential points and the proper practices for measuring low concentration biomolecule samples and supporting LOD claims.