Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces.
Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces.
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DOI：
10.1007/978-1-62703-317-6_5
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Niedernhofer, Laura J
中科院分区：
文献类型：
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作者：
Lavasani, Mitra;Lu, Aiping;Thompson, Seth D;Robbins, Paul D;Huard, Johnny;Niedernhofer, Laura J
我们的实验室开发并优化了一种方法，称为改进的预板技术，从骨骼肌中分离干细胞/祖细胞。这种方法根据不同群体的肌源性细胞粘附于胶原i包被表面的倾向来分离它们。基于它们的表面标记物和干细胞样特性，包括自我更新、多系分化和促进组织再生的能力，最后一个或最慢粘附到胶原包覆表面的细胞部分（pre-plate 6; pp6）似乎是早期的、静止的祖细胞，称为肌源性干细胞/祖细胞（mdspc）。pp6之前的细胞片段（pp1-5）可能是更稳定（分化）的细胞群，包括成纤维细胞和成肌细胞样细胞。该技术可用于从人类或小鼠的骨骼肌中分离MDSPCs，无论其年龄、性别或疾病状态如何，尽管MDSPCs的产量随年龄和健康状况而变化。MDSPCs可用于多种组织的再生，包括骨、关节软骨、骨骼肌、心肌和神经。MDSPCs目前正在临床试验中用于治疗尿失禁和心肌梗死。在加速衰老的小鼠模型中，来自年轻小鼠的MDSPCs也被证明通过明显的旁分泌/内分泌机制延长寿命和健康寿命。本文详细介绍了mdspc的分离和表征方法。
Our lab developed and optimized a method, known as the modified pre-plate technique, to isolate stem/progenitor cells from skeletal muscle. This method separates different populations of myogenic cells based on their propensity to adhere to collagen I-coated surface. Based on their surface markers and stem-like properties, including self-renewal, multi-lineage differentiation, and ability to promote tissue regeneration, the last cell fraction or slowest to adhere to the collagen-coated surface (pre-plate 6; pp6) appear to be early, quiescent progenitor cells termed muscle-derived stem/progenitor cells (MDSPCs). The cell fractions preceding pp6 (pp1–5) are likely populations of more committed (differentiated) cells, including fibroblast- and myoblastlike cells. This technique may be used to isolate MDSPCs from skeletal muscle of humans or mice regardless of age, sex or disease state, although the yield of MDSPCs varies with age and health. MDSPCs can be used for regeneration of a variety of tissues including bone, articular cartilage, skeletal and cardiac muscle and nerve. MDSPCs are currently being tested in clinical trials for treatment of urinary incontinence and myocardial infarction. MDSPCs from young mice have also been demonstrated to extend lifespan and healthspan in mouse models of accelerated aging through an apparent paracrine/endocrine mechanism. Here we detail methods for isolation and characterization of MDSPCs.