Fibroblast growth factor-2 and vascular endothelial growth factor mediated augmentation of angiogenesis and bone formation in vascularized bone allotransplants.
Fibroblast growth factor-2 and vascular endothelial growth factor mediated augmentation of angiogenesis and bone formation in vascularized bone allotransplants.
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成纤维细胞生长因子-2 和血管内皮生长因子介导血管化同种异体骨移植中血管生成和骨形成的增强。
DOI：
10.1002/micr.22221
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发表时间：
2014
期刊：
影响因子：
2.1
通讯作者：
Bishop,AllenT
中科院分区：
文献类型：
--
作者：
Larsen,Mikko;Willems,WouterF;Pelzer,Michael;Friedrich,PatriciaF;Dadsetan,Mahrokh;Bishop,AllenT
我们之前证明了受体源性新血管生成可以在没有长期免疫抑制的情况下维持活骨同种异体移植物的活力。研究了细胞因子递送对增强该过程的作用。从Dark Agglomerate到Piebald Virol Glaxo大鼠进行血管化股骨移植。将载有缓冲液（N= 11）、碱性成纤维细胞生长因子（FGF 2）（N= 10）、血管内皮生长因子（VEGF）（N= 11）或两者（N= 11）的聚（d，l-丙交酯-共-乙交酯）微球沿受体衍生动静脉束插入髓内。FK-506给药2周。在18周时，进行骨血流量、微血管造影、组织学、组织形态学和碱性磷酸酶测量。联合组骨血流量高于对照组和VEGF组（P= 0.04）。FGF 2组毛细血管密度高于VEGF组和联合组（P< 0.05）。骨活力、生长和碱性磷酸酶活性在组间无显著差异。血管化骨同种异体移植物中的新血管生成通过血管生成细胞因子递送而增强，使用FGF 2的结果与来自先前研究的同种移植物相当。需要进一步的研究来实现类似于同种移植物的骨形成。© 2014 Wiley Periodicals，Inc.显微外科34：301-307，2014年。
We previously demonstrated recipient‐derived neoangiogenesis to maintain viability of living bone allogeneic transplants without long‐term immunosuppression. The effect of cytokine delivery to enhance this process is studied. Vascularized femur transplantation was performed from Dark Agouti to Piebald Virol Glaxo rats. Poly(d,l‐lactide‐co‐glycolide) microspheres loaded with buffer (N= 11), basic fibroblast growth factor (FGF2) (N= 10), vascular endothelial growth factor (VEGF) (N= 11), or both (N= 11) were inserted intramedullarly alongside a recipient‐derived arteriovenous bundle. FK‐506 was administered for 2 weeks. At 18 weeks, bone blood flow, microangiography, histologic, histomorphometric, and alkaline phosphatase measurements were performed. Bone blood flow was greater in the combined group than control and VEGF groups (P= 0.04). Capillary density was greater in the FGF2 group than in the VEGF and combined groups (P< 0.05). Bone viability, growth, and alkaline phosphatase activity did not vary significantly between groups. Neoangiogenesis in vascularized bone allotransplants is enhanced by angiogenic cytokine delivery, with results using FGF2 that are comparable to isotransplant from previous studies. Further studies are needed to achieve bone formation similar to isotransplants. © 2014 Wiley Periodicals, Inc. Microsurgery 34:301–307, 2014.