A cytoplasmic high affinity estrogen-binding protein in the embryonic chicken liver.
A cytoplasmic high affinity estrogen-binding protein in the embryonic chicken liver.
复制标题
胚胎鸡肝中的细胞质高亲和力雌激素结合蛋白。
DOI：
10.1111/j.1432-1033.1977.tb11901.x
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
W. Kittstein
中科院分区：
文献类型：
--
作者：
M. Gschwendt;W. Kittstein
1.鸡胚肝含有高亲和力(Kd=1×10~(-9)M)和低容量(200-1100个结合部位/细胞，取决于胚龄)的细胞质雌激素结合部位。2.只有雌激素与高亲和力结合。3.至少这些结合部位的雌激素结合部分是一种蛋白质。4.经琼脂糖凝胶层析，测得表观分子量为50000-60000道尔顿，与核雌激素结合蛋白非常相似。5.在低盐条件下，胞质结合位点具强烈的聚集倾向，但可被温和的胰酶消化所消除。6.单次向卵子注射雌二醇似乎会导致细胞质结合蛋白移位到细胞核。7.在胚胎发育过程中，结合位点数一直增加到19日龄。此后，它再次下降，在孵化后不久达到一个恒定的低水平。8.提供的数据表明，这种低水平的结合位点不可能完全是由于孵化动物肝脏匀浆中最终存在的一种成分在体外降解或抑制。
1. Embryonic chicken liver contains cytoplasmic estrogen-binding sites with high affinity for estradiol (Kd = 1 X 10(-9) M) and with low capacity (200-1100 binding sites/cell, dependeing on the age of the embryo). 2. Only estrogenic hormones are bound with high affinity. 3. At least the estrogen-bindin moiety of these binding sites is a protein. 4. By agarose gel chromatography an apparent molecular weight of 50000-60000 daltons was determined, very similar to that of the nuclear estrogen-binding protein. 5. In low salt the cytoplasmic binding sites exhibit a strong tendency to aggregate, which is abolished by mild trypsination. 6. A single injection of estradiol into the egg seems to cause a translocation of the cytoplasmic binding protein to the nucleus. 7. During the embryonic development the number of binding sites increases up to the age of 19 days. Thereafter it decreases again reaching a constant low level soon after hatching. 8. Data are presented to show that this low level of binding sites can not be due solely to a degradation or inhibition in vitro by a component eventually present in the liver homogenate of hatched animals.