The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development
The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development
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DOI：
10.1177/154405910308201003
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发表时间：
2003-10-01
影响因子：
7.6
通讯作者：
Mishina, Y
中科院分区：
文献类型：
--
作者：
Feng, JQ;Huang, H;Mishina, Y
牙本质基质蛋白I（Dentin Matrix Protein I，Dmp 1）最初是从牙本质中发现的。然而，其在体内的表达和功能尚不清楚。为了澄清这两个问题，我们已经产生了小鼠携带截短的Dmp 1基因，通过使用基因靶向取代外显子6与lacZ基因。北方印迹分析显示预期的5.8-kb Dmp 1-lacZ融合转录物和野生型2.8-kb Dmp 1转录物的丢失，通过缺乏蛋白质的免疫染色证实。使用杂合子动物，我们证明，Dmp 1是具体的矿化组织。以前没有显示，Dmp 1也在牙髓细胞中表达。dmp 1缺陷的胚胎和新生儿没有表现出明显的总体异常表型，尽管肥大软骨细胞区适度扩张，长骨直径适度增加。这表明DMPI对于早期小鼠骨骼或牙齿发育不是必需的。
Dentin Matrix Protein I (Dmp1) was originally identified from dentin. However, its expression and function in vivo are not clear. To clarify these two issues, we have generated mice carrying a truncated Dmp1 gene by using gene targeting to replace exon 6 with a lacZ gene. Northern blot analysis shows the expected 5.8-kb Dmp1-lacZ fusion transcript and loss of the wild-type 2.8-kb Dmp1 transcript, confirmed by a lack of immunostaining for the protein. Using heterozygous animals, we demonstrate that Dmp1 is specific for mineralized tissues. Not previously shown, Dmp1 is also expressed in pulp cells. Dmp1-deficient embryos and newborns display no apparent gross abnormal phenotype, although there are a modest expansion of the hypertrophic chondrocyte zone and a modest increase in the long bone diameter. This suggests that DMPI is not essential for early mouse skeletal or dental development.