Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes
Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes
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DOI：
10.1038/nature02069
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发表时间：
2003-10-30
期刊：
影响因子：
64.8
通讯作者：
Alvarez-Buylla, A
中科院分区：
文献类型：
--
作者：
Alvarez-Dolado, M;Pardal, R;Alvarez-Buylla, A
近期研究表明，骨髓细胞具有广泛的分化潜能，能够形成新的肝细胞、心肌细胞和神经元（1,2）。一些研究小组将这种明显的可塑性归因于“转分化”（3 - 5）。然而，其他研究小组则提出细胞融合可以解释这些结果（6 - 9）。我们使用一种基于Cre/lox重组的简单方法来检测细胞融合事件，结果证明骨髓来源的细胞（BMDCs）在体外可与神经祖细胞自发融合。此外，骨髓移植实验表明，BMDCs在体内可与肝脏中的肝细胞、大脑中的浦肯野神经元以及心脏中的心肌细胞融合，从而形成多核细胞。在这些组织中未观察到无融合的转分化证据。这些观察结果首次提供了BMDCs与神经元和心肌细胞在体内融合的证据，这增加了细胞融合可能有助于这些关键细胞类型的发育或维持的可能性。
Recent studies have suggested that bone marrow cells possess a broad differentiation potential, being able to form new liver cells, cardiomyocytes and neurons(1,2). Several groups have attributed this apparent plasticity to 'transdifferentiation'(3-5). Others, however, have suggested that cell fusion could explain these results(6-9). Using a simple method based on Cre/lox recombination to detect cell fusion events, we demonstrate that bone-marrow-derived cells (BMDCs) fuse spontaneously with neural progenitors in vitro. Furthermore, bone marrow transplantation demonstrates that BMDCs fuse in vivo with hepatocytes in liver, Purkinje neurons in the brain and cardiac muscle in the heart, resulting in the formation of multinucleated cells. No evidence of transdifferentiation without fusion was observed in these tissues. These observations provide the first in vivo evidence for cell fusion of BMDCs with neurons and cardiomyocytes, raising the possibility that cell fusion may contribute to the development or maintenance of these key cell types.