The Effect of Aloe Vera Oral Administration on Cutaneous Wound Healing in Type 2 Diabetic Rats
The Effect of Aloe Vera Oral Administration on Cutaneous Wound Healing in Type 2 Diabetic Rats
复制标题
DOI：
10.1292/jvms.10-0438
复制
发表时间：
2011-05-01
影响因子：
1.2
通讯作者：
Uzuka, Yuji
中科院分区：
文献类型：
--
作者：
Atiba, Ayman;Ueno, Hiroshi;Uzuka, Yuji
伤口延迟愈合是糖尿病的并发症之一。本研究旨在探讨芦荟口服给药对2型糖尿病大鼠开放性伤口的影响。在全身麻醉下，在大鼠背部形成全层开放性伤口（1.5 x 1.5 cm）。将这些大鼠分为两组，对照组（C组）和芦荟口服给药组（A组）。在伤后第1、2、4和8天测量每个伤口面积。组织病理学评价创面肉芽组织分期。免疫组化法检测转化生长因子（TGF）-β 1和血管内皮生长因子（VEGF）的表达。A组伤后2、4、8天创面明显收缩。组织学结果显示，A组炎症细胞浸润、血管生成、细胞外基质沉积和上皮化均明显增强。免疫组化结果显示，伤后第4天，A组TGF-β 1和VEGF蛋白阳性细胞增加。我们得出结论，芦荟口服给药加速2型糖尿病大鼠伤口愈合。
Delayed wound healing is one of the complications of diabetes mellitus. The present study was performed to investigate the effect of Aloe vera oral administration on open wounds in type 2 diabetic rats. Full thickness open wounds (1.5 x 1.5 cm) were created under general anesthesia on the backs of the rats. These rats were divided into two group, a control group (Group C) and an Aloe vera oral administration group (Group A). Each wound area was measured on days 1, 2, 4 and 8 postwounding. The stages of wound granulation tissues were evaluated histopathologically. The expression of transforming growth factor (TGF)-beta 1 and vascular endothelial growth factor (VEGF) were determined by immunohistochemically. The wounds were significantly contracted in Group A on days 2, 4 and 8 postwounding. Histological results revealed that the inflammatory cell infiltration, angiogenesis, extracellular matrix deposition and epithelialization were promoted in Group A, respectively. The immunohistochemical results revealed that both TGF-beta 1 and VEGF protein-positive cells increased in Group A on day 4 postwounding. We concluded that Aloe vera oral administration accelerated wound healing in type 2 diabetic rats.