Kaposi's sarcoma-associated herpesvirus and Kaposi's sarcoma in Africa. Uganda Kaposi's Sarcoma Study Group.
Kaposi's sarcoma-associated herpesvirus and Kaposi's sarcoma in Africa. Uganda Kaposi's Sarcoma Study Group.
复制标题
卡波西肉瘤相关疱疹病毒和非洲的卡波西肉瘤。
作者：
Yuan Chang;J. Ziegler;H. Wabinga;E. Katangole;C. Boshoff;T. Schulz;D. Whitby;Dawn Maddalena;H. Jaffe;R. Weiss;P. Moore
背景
地方性卡波西肉瘤（KS）是发生在非洲的一种临床和流行病学上独特的人类免疫缺陷病毒阴性形式的KS。卡波西肉瘤现在是非洲一些地区最常报告的癌症。
目的
确定KS相关疱疹病毒（KSHV）是否存在于非洲患者的地方性HIV血清阴性和HIV血清阳性KS病变中。
方法
在一项盲法病例对照研究中，对来自乌干达KS患者和非KS肿瘤对照患者的石蜡包埋组织标本进行了检查。在两个独立的实验室通过巢式聚合酶链反应检测组织DNA标本中可检测的KSHV基因组。
结果
我们确定KSHV在17（85%）的20 KS组织标本从HIV血清阴性患者和22（92%）的24 KS组织标本从HIV感染者。4例HIV感染者和4例HIV血清阴性者的卡波西肉瘤病变均为KSHV阳性。与之前在北美和欧洲的研究不同，22例非KS癌症对照患者的组织标本中有3例（14%）也为KSHV阳性，这导致乌干达患者KS病变中检测KSHV的总体比值比为49.2（95%置信区间，9.1至335）。
结论
与北美和欧洲一样，KSHV感染与非洲的HIV血清阳性和HIV血清阴性KS密切相关。然而，这种病毒的感染可能在乌干达更为普遍。
BACKGROUND
Endemic Kaposi's sarcoma (KS) is a clinically and epidemiologically distinct human immunodeficiency virus negative form of KS occurring in Africa. Kaposi's sarcoma is now the most frequently reported cancer in some areas of Africa.
OBJECTIVE
To determine if a KS-associated herpesvirus (KSHV) is present in both endemic HIV-seronegative and HIV-seropositive KS lesions from African patients.
METHODS
Paraffin-embedded tissue specimens from Ugandan patients with KS and non-KS tumor control patients attending a university-based oncology clinic were examined in a blinded case-control study. Tissue DNA specimens were examined for detectable KSHV genome by nested polymerase chain reaction performed at two independent laboratories.
RESULTS
We identified KSHV in 17 (85%) of 20 KS tissue specimens from HIV-seronegative patients and 22 (92%) of 24 KS tissue specimens from HIV-infected persons. Kaposi's sarcoma lesions from four HIV-infected persons and four HIV-seronegative persons were positive for KSHV. Unlike previous studies in North America and Europe, three (14%) of 22 non-KS cancer control patients' tissue specimens were also positive for KSHV that resulted in an overall odds ratio of 49.2 (95% confidence interval, 9.1 to 335) for detecting KSHV in KS lesions from patients in Uganda.
CONCLUSION
As in North America and Europe, KSHV infection is strongly associated with both HIV-seropositive and HIV-seronegative KS in Africa. However, it is likely that infection with this virus is more highly prevalent in Uganda.