Foot-and-mouth disease type O viruses exhibit genetically and geographically distinct evolutionary lineages (topotypes)
Foot-and-mouth disease type O viruses exhibit genetically and geographically distinct evolutionary lineages (topotypes)
复制标题
DOI：
10.1099/0022-1317-82-3-609
复制
发表时间：
2001-03-01
影响因子：
3.8
通讯作者：
Knowles, NJ
中科院分区：
文献类型：
--
作者：
Samuel, AR;Knowles, NJ
O型是口蹄疫病毒七种血清型中最流行的一种，在世界许多地方都有发生。采用UPGMA法对105株O型口蹄疫病毒VPI基因3'端核苷酸序列进行系统进化树分析。当15%核苷酸差异的值用作截止值时，该分析鉴定了8种主要基因型。这些分组的有效性进行了测试的完整的VP1基因序列的23个这些病毒的bootstrap restering和建设的邻居加入树。这八个遗传谱系属于地理界限，我们用拓扑型一词来描述它们。使用一个大的序列数据库，属于每个八个拓扑型的病毒的分布已被确定。这些基于遗传学的流行病学研究也被用来确定已经超越其正常生态位的病毒。尽管口蹄疫病毒基因组复制过程中突变率很高，但拓扑型似乎代表了进化的死胡同。
Serotype O is the most prevalent of the seven serotypes of foot-and-mouth disease (FMD) virus and occurs in many parts of the world. The UPGMA method was used to construct a phylogenetic tree based on nucleotide sequences at the 3' end of the VPI gene from 105 FMD type O viruses obtained from samples submitted to the OIE/FAO World Reference Laboratory for FMD. This analysis identified eight major genotypes when a value of 15% nucleotide difference was used as a cut-off. The validity of these groupings was tested on the complete VP1 gene sequences of 23 of these viruses by bootstrap resampling and construction of a neighbour-joining tree. These eight genetic lineages fell within geographical boundaries and we have used the term topotype to describe them. Using a large sequence database, the distribution of viruses belonging to each of the eight topotypes has been determined. These phylogenetically based epidemiological studies have also been used to identify viruses that have transgressed their normal ecological niches. Despite the high rate of mutation during replication of the FMD virus genome, the topotypes appear to represent evolutionary cul-de-sacs.