Isoflavonoid production by adventitious-root cultures of Iris germanica (Iridaceae)
Isoflavonoid production by adventitious-root cultures of Iris germanica (Iridaceae)
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DOI：
10.5511/plantbiotechnology.22.207
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发表时间：
2005-09
影响因子：
1.6
通讯作者：
T. Akashi;Masayuki Ishizaki;T. Aoki;S. Ayabe
中科院分区：
文献类型：
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作者：
T. Akashi;Masayuki Ishizaki;T. Aoki;S. Ayabe
我们建立了德国蝴蝶花的不定根培养体系，这是一种单子叶植物，以生产异黄酮类化合物而闻名。Irigenin(5，7，3′-trihydroxy-6，4′，5′-trimethoxyisoflavone)和Iristectorigin A(5，7，3‘-三羟基-6，4’-二甲氧基异黄酮)及其7-O-β-D-葡萄糖苷、Irigectorin A和Iristectorin A分别是液体培养中不定根的主要成分，培养3周时，总异黄酮量为每克鲜重3.6微克摩尔，远高于此前报道的每克根茎干重0.70微克摩尔(Ali等人)。1983年植物化学22：2061)。在液体培养基中加入3 mM氯化铜(CuCl2)，施加非生物胁迫。异黄酮苷含量在CuCl2处理的最初6h内呈上升趋势，随后下降；苷元水平在整个处理48h内持续上升。在6~48h内，糖苷含量的下降与苷元含量的增加呈负相关，在CuCl2处理后6h的总异黄酮苷+苷元含量是初始值的1.4倍，48h内几乎保持不变，因此，CuCl2处理的主要作用似乎是诱导异黄酮苷的水解。克隆了查尔酮合酶基因，并在产生异黄酮的培养物中进行了表达。
We established the adventitious-root cultures of Iris germanica, a monocotyledonous plant known for isoflavonoid production. Irigenin (5,7,3′-trihydroxy-6,4′,5′-trimethoxyisoflavone) and iristectorigenin A (5,7,3′-trihydroxy-6,4′-dimethoxyisoflavone) along with their 7-O-β-D-glucosides, iridin and iristectorin A, respectively, were found as the major components in adventitious roots in the liquid medium, and the total isoflavone content was about 3.6 µmol per g fresh weight in 3-week-old cultures, which was much higher than the 0.7 µmol per g dry weight in the rhizome previously reported (Ali et al. 1983 Phytochemistry 22: 2061). Abiotic stress was applied by addition of 3 mM cupric chloride (CuCl2) to the liquid medium. The isoflavone glucoside content was increased during the initial 6 h of CuCl2 treatment, which was followed by a decrease; the aglycone level continued to increase throughout the 48 h of treatment. The decrease in glucoside content was negatively correlated with the increase in aglycone content between 6 and 48 h. The total isoflavone content (glucosides+aglycones) at 6 h after the start of CuCl2 treatment was 1.4-fold the initial value, and nearly the same content was maintained for 48 h. Thus, the main effect of CuCl2 treatment appeared to be the induction of hydrolysis of isoflavone glucosides. A cDNA of chalcone synthase was cloned, and the mRNA was expressed in the culture producing isoflavones.