STRUCTURE OF THE REACTION CENTER FROM RHODOBACTER-SPHAEROIDES R-26 - THE PROTEIN SUBUNITS
STRUCTURE OF THE REACTION CENTER FROM RHODOBACTER-SPHAEROIDES R-26 - THE PROTEIN SUBUNITS
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DOI：
10.1073/pnas.84.17.6162
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发表时间：
1987-09-01
影响因子：
11.1
通讯作者：
REES, DC
中科院分区：
文献类型：
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作者：
ALLEN, JP;FEHER, G;REES, DC
用X射线衍射法测定了类球红细菌反应中心（RC）蛋白质亚基的三维结构，其分辨率为2.8埃。R系数为26%。L和M亚基各自含有五个跨膜螺旋和几个不跨越膜的螺旋。L亚基和M亚基通过2重旋转对称轴彼此相关，该旋转对称轴与辅因子所确定的旋转对称轴大致相同。H亚基具有一个跨膜螺旋和在细胞质侧的球状结构域，其含有不跨越膜的螺旋和几个β-环。薄膜.与其他紫色细菌的RC的结构同源性进行了讨论。水溶性细胞色素c2和Rb的RC之间形成的复合物的结构。sphaeroides提出。
The three-dimensional structure of the protein subunits of the reaction center (RC) of Rhodobacter sphaeroides has been determined by x-ray diffraction at a resolution of 2.8 .ANG. with an R factor of 26%. The L and M subunits each contain five transmembrane helices and several helices that do not span the membrane. The L and M subunits are related to each other by a 2-fold rotational symmetry axis that is approximately the same as that determined for the cofactors. The H subunit has one transmembrane helix and a globular domain on the cytoplasmic side, which contains a helix that does not span the membrane and several .beta.-sheets. The structured homology with RCs from other purple bacteria is discussed. A structure of the complex formed between the water soluble cytochrome c2 and the RC from Rb. sphaeroides is proposed.