Long term survival of allografted muscle precursor cells following a limited period of treatment with cyclosporin A.
Long term survival of allografted muscle precursor cells following a limited period of treatment with cyclosporin A.
复制标题
用环孢菌素 A 进行有限时间的治疗后，同种异体移植的肌肉前体细胞可长期存活。
DOI：
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发表时间：
1984
影响因子：
4.6
通讯作者：
T. Partridge
中科院分区：
文献类型：
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作者：
D. Watt;J. Morgan;T. Partridge
先前的工作（Watt etal ., 1982）表明，在小鼠中，骨骼肌可以以其单核细胞前体的悬浮形式成功移植。通过将来源于正常肌肉的前体细胞移植到肌病患者体内，这项技术的最终治疗应用将需要一种适用于人类的防止同种异体移植排斥反应的方法。因此，我们研究了使用药物环孢素A （CyA）作为延长同种异体单核肌细胞移植到再生宿主肌肉区域的小鼠存活的手段。从嫁接之日起，我们以75或150 mg/kg体重/天的剂量给宿主CyA，持续42天。通过使用同种异体酶作为宿主和供体组织的标记，我们已经证明同种异体移植的单核细胞可以融入宿主肌纤维，并且这样形成的嵌合宿主/供体肌纤维可以在我们继续实验的时间内存活，移植后最多107天，或CyA处理结束后65天。
Previous work (Watt et al., 1982) has shown that, in the mouse, skeletal muscle can be transplanted successfully in the form of a suspension of it's mononucleate precursors. Eventual therapeutic application of this technique by the implantation of precursor cells derived from normal muscle into myopathic individuals would require a means of preventing allograft rejection applicable to man. We have therefore investigated the use of the drug cyclosporin A (CyA) as a means of prolonging the survival in mice of allografts of mononucleate muscle cells made into a region of regenerating host muscle. We have administered CyA to the hosts at doses of either 75 or 150 mg/kg body weight/day for 42 days from the day of grafting. By using isoenzyme allotypes as markers of host and donor tissues, we have shown that allografted mononucleate cells become incorporated in host muscle fibres and that the mosaic host/donor muscle fibres so formed survive for as long as we continued the experiment, a maximum of 107 days after grafting, or 65 days after the end of CyA treatment.