Multiplexed ion mobility spectrometry-orthogonal time-of-flight mass spectrometry
Multiplexed ion mobility spectrometry-orthogonal time-of-flight mass spectrometry
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DOI：
10.1021/ac0617316
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发表时间：
2007-03-15
影响因子：
7.4
通讯作者：
Smith, Richard D.
中科院分区：
文献类型：
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作者：
Belov, Mikhail E.;Buschbach, Michael A.;Smith, Richard D.
离子迁移谱（IMS）耦合正交飞行时间质谱（TOF）已显示出显着的承诺，为复杂的生物混合物的表征。生物样品的巨大复杂性（例如，来自蛋白质组学）以及对生物学和技术分析重复的需要对多维分离平台在灵敏度和样品通量方面提出了重大挑战。IMS-TOF MS平台的一个主要潜在吸引力是分离速度超过常规凝相分离的数量级。目前减轻这种吸引力的IMS-TOF MS平台的已知限制包括由于包括在IMS-TOF界面中的显著离子损失和在连续离子源的情况下小于类似于1%的离子利用效率（例如，ESI）。我们开发了一种新型的多路复用ESI-IMS-TOF质谱仪，该质谱仪能够实现通过IMS-TOF的无损离子传输，并且对来自ESI源的离子的利用效率> 50%。肽混合物的初始结果显示，与信号平均方法相比，多重方法的信噪比增加了10倍，IMS或TOF MS分辨率均未降低。
Ion mobility spectrometry (IMS) coupled to orthogonal time-of-flight mass spectrometry (TOF) has shown significant promise for the characterization of complex biological mixtures. The enormous complexity of biological samples (e.g., from proteomics) and the need for both biological and technical analysis replicates imposes major challenges for multidimensional separation platforms with regard to both sensitivity and sample throughput. A major potential attraction of the IMS-TOF MS platform is separation speeds exceeding that of conventional condensed-phase separations by orders of magnitude. Known limitations of the IMS-TOF MS platforms that presently mitigate this attraction include the need for extensive signal averaging due to factors that include significant ion losses in the IMS-TOF interface and an ion utilization efficiency of less than similar to 1% with continuous ion sources (e.g., ESI). We have developed a new multiplexed ESI-IMS-TOF mass spectrometer that enables lossless ion transmission through the IMS-TOF as well as a utilization efficiency of > 50% for ions from the ESI source. Initial results with a mixture of peptides show a similar to 10-fold increase in signal-to-noise ratio with the multiplexed approach compared to a signal averaging approach, with no reduction in either IMS or TOF MS resolution.