Suppressor of cytokine signaling 1 gene expression and polymorphisms in systemic lupus erythematosus
Suppressor of cytokine signaling 1 gene expression and polymorphisms in systemic lupus erythematosus
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DOI：
10.1177/0961203309357437
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发表时间：
2010-05-01
期刊：
影响因子：
2.6
通讯作者：
Yen, J. H.
中科院分区：
文献类型：
--
作者：
Chan, H. C.;Ke, L. Y.;Yen, J. H.
为探讨细胞因子信号转导抑制因子1(SOCS1)在系统性红斑狼疮发病机制中的作用，对107例系统性红斑狼疮患者、101例健康对照和151例强直性脊柱炎患者进行了研究。采用实时定量聚合酶链式反应方法检测SOCS1基因表达水平。采用聚合酶链式反应/限制性片段长度多态方法检测SOCS1基因多态性。用SLEDAI评分系统评价系统性红斑狼疮的疾病活动性。结果表明，系统性红斑狼疮患者外周血中SOCS1mRNA的表达明显高于正常对照组(p=0.0014)。系统性红斑狼疮活动期患者SOCS1mRNA的表达明显高于非活动期患者(P=0.035)。系统性红斑狼疮患者、健康对照组和强直性脊柱炎患者SOCS1-1478CA/del基因多态性频率差异无统计学意义。在显性模式(CA/del+del/del vs CA/CA)中，有血小板减少的患者SOCS1-1478多态的基因频率明显低于无血小板减少的患者(p(C)=0.035)。此外，有血小板减少者SOCS1-1478del等位基因频率也显著低于无血小板减少者(p(C)=0.02)。综上所述，本研究证实系统性红斑狼疮患者外周血中SOCS1基因表达水平显著升高。系统性红斑狼疮活动期患者SOCS1mRNA水平显著高于非活动期患者。我们还发现有血小板减少的系统性红斑狼疮患者的SOCS1-1478del频率低于无血小板减少的患者。狼疮(2010)19,696-702。
With the aim of investigating the role of suppressor of cytokine signaling 1 (SOCS1) in the pathogenesis of systemic lupus erythematosus, 107 patients with systemic lupus erythematosus, 101 healthy controls, and 151 patients with ankylosing spondylitis were enrolled in this study. SOCS1 mRNA level was measured by the method of quantitative real-time polymerase chain reaction. SOCS1 polymorphisms were detected by the polymerase chain reaction/restriction fragment length polymorphisms method. Systemic lupus erythematosus disease activity was evaluated with the SLEDAI. This study showed that the SOCS1 mRNA expression was significantly higher in the patients with systemic lupus erythematosus than in the healthy controls (p = 0.0014). Patients with active systemic lupus erythematosus had a higher expression of SOCS1 mRNA than the patients with inactive systemic lupus erythematosus (p = 0.035). There was no significant difference in the frequencies of the SOCS1-1478CA/del polymorphisms among the patients with systemic lupus erythematosus, healthy controls, and patients with ankylosing spondylitis. The genotype frequency of the SOCS1-1478 polymorphisms in the dominant model (CA/del+del/del versus CA/CA) was significantly decreased in the patients with thrombocytopenia compared with those without thrombocytopenia (p(c) = 0.035). Moreover, the allele frequency of SOCS1-1478del was also significantly lower in the patients with thrombocytopenia than in those without thrombocytopenia (p(c) = 0.02). In conclusion, this study demonstrated that the expression of SOCS1 mRNA was significantly increased in patients with systemic lupus erythematosus. Moreover, SOCS1 mRNA levels in patients with active systemic lupus erythematosus were significantly higher than those in the inactive patients. We also found that the systemic lupus erythematosus patients with thrombocytopenia have a lower frequency of SOCS1-1478del compared with patients without thrombocytopenia. Lupus (2010) 19, 696-702.