CRTCI/MAML2 fusion transcript in Warthin's tumor and Mucoepidermoid carcinoma: Evidence for a common genetic association
CRTCI/MAML2 fusion transcript in Warthin's tumor and Mucoepidermoid carcinoma: Evidence for a common genetic association
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DOI：
10.1002/gcc.20534
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发表时间：
2008-04-01
影响因子：
3.7
通讯作者：
El-Naggar, Adel K.
中科院分区：
文献类型：
--
作者：
Bell, Diana;Luna, Mario A.;El-Naggar, Adel K.
易位和基因融合在肿瘤发生中具有重要的早期作用。t（11; 19）易位及其CRTCII MAML 2融合转录物已经在沃辛肿瘤和粘液表皮样癌的几个实例中鉴定，并且被认为与这些肿瘤的一个子集的发展相关。为了确定沃辛瘤和粘液表皮样癌是否有遗传相关性，我们使用逆转录-聚合酶链反应和DNA测序分析了沃辛瘤和粘液表皮样癌组成的三个肿瘤的显微解剖成分。我们还调查了转移性黑色素瘤沃辛瘤和沃辛癌的腮腺进行比较。在沃辛肿瘤和所有三种肿瘤的匹配粘液表皮样癌组分中，在沃辛癌中，以及在沃辛肿瘤组分中鉴定出融合转录物，但在转移性黑色素瘤中没有。这些结果为t（11; 19）融合基因与伴有粘液表皮样癌的沃辛肿瘤亚群的发展以及可能向沃辛癌的恶性转化之间的联系提供了证据。
Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTCII MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and mucoepidermoid carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and mucoepidermoid carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and mucoepidermoid carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching mucoepidermoid carcinoma components of all three tumors, in the Warthin's carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11; 19) fusion gene and the development of a subset of Warthin's tumors with concurrent mucoepidermoid carcinoma and possible malignant transformation to Warthin's carcinoma.