SUBSTRATE-SPECIFICITY OF CHONDROSULFATASES FROM PROTEUS-VULGARIS FOR SULFATED TETRASACCHARIDES
SUBSTRATE-SPECIFICITY OF CHONDROSULFATASES FROM PROTEUS-VULGARIS FOR SULFATED TETRASACCHARIDES
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DOI：
10.1016/0304-4165(74)90221-9
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
ANNO, K
中科院分区：
文献类型：
--
作者：
SENO, N;AKIYAMA, F;ANNO, K
1. Three novel sulfated tetrasaccharides, III, IV and V were isolated from the digestion products of chondroitin sulfate C, E and K, with testicular hyaluronidase, and were characterized as follows: III, O-(β-D-glucopyranosyluronic acid)-(1→ 3)-O-2 acetamido-2-deoxy-6-O-sulfo-β-D-gala ctopyranosyl-(1→ 4)-O-(β-D-glucopyranosyluronicacid)-(1→ 3)-2-acetamido-2-deoxy-4-O-sulfo-D-galactose; IV, O-(β-D-glucopyranosyluronicacid)-(1→ 3)-O-2-acetamido-2-deoxy-4, 6-di-O-sulfo-β-D-galactopyranosyl-(1→ 4)-O-(β-D-glucopyranosyluronicacid)-(1→ 3)-2-acetamido-2-deoxy-4-O-sulfo-D-galactose; V, O-(2-or3-O-sulfo-β-D-glucopyranosyluronic acid)-(1→ 3)-O-2-acetamido-2-deoxy-4-O-sulfo-β-D-galactopyranos yl-(1→ 4)-O-(β-D-glucopyranosyluronicacid)-(1→ 3)-2-acetamido-2-deoxy-4-O-sulfo-D-galactose. 2. 2. Effects of chondro-4-and-6-sulfatases of Proteus vulgaris were studied on III, IV, V, in addition to the tetrasaccharides I, O-(β-D-glucopyranosyluronic acid)-(1→ 3)-O-2-acetamido-2-deoxy-4-O-sulfo-β-D-galactopyranosyl-(1→ 4)-O-(β-D-glucopyranosyluronicacid)-(1→ 3)-2-acetamido-2-deoxy-4-O-sulfo-D-galactose and II, O-(β-D-glucopyranosyluronic acid)-(1→ 3)-O-2-acetamido-2-deoxy-6-O-sulfo-β-D-galactopyranosyl-(1→ 4)-O (β-D-glucopyranosyluronicacid)-(1→ 3)-2-acetamido-2-deoxy-6-O-sulfo-D-galactose. 3. 3. The chondro-4-sulfatase attacked preferentially the ester sulfate on the C-4 of the N-acetylgalactosamine residue in the reducing end of the tetrasaccharide I, III, IV, V, regardless of the positions of the ester sulfates in the non-reducing disaccharide moieties, and then gradually attacked the ester sulfate on the C-4 of the other side in I and V. On the other hand, chondro-6-sulfatase scarcely affected the C-6 ester sulfate of sulfated tetrasaccharides.
