Effects of 5-azacytidine on the progressive nature of cell transformation.
Effects of 5-azacytidine on the progressive nature of cell transformation.
复制标题
5-氮杂胞苷对细胞转化进行性的影响。
DOI：
10.1128/mcb.5.7.1800-1803.1985
复制
发表时间：
1985
影响因子：
5.3
通讯作者：
Weinstein,IB
中科院分区：
文献类型：
--
作者：
Hsiao,WL;Gattoni-Celli,S;Weinstein,IB
将C3 H 10 T1/2小鼠胚胎成纤维细胞暴露于3 μM 5-氮杂胞苷24 h，然后在不存在5-氮杂胞苷的情况下连续传代，并检查生长特性的后续变化。经处理的细胞在形态、饱和密度、生长速率和血清依赖性方面表现出变化。到第5代，它们获得在0.3%琼脂糖中生长的能力，到第30代，它们产生在琼脂糖、琼脂和液体悬浮液中生长的完全转化的病灶。如果将5-氮杂胞苷处理的细胞培养物暴露于致癌物苯并[a]芘48 h，则这种进展迅速加速。这些研究的结果提供了证据，DNA甲基化的异常可能是一系列的关键事件在多阶段的癌变过程中，从而促进肿瘤细胞的演变。
C3H 10T1/2 mouse embryo fibroblasts were exposed to 3 μM 5-azacytidine for 24 h and then serially passaged in the absence of 5-azacytidine and examined for subsequent changes in growth properties. The treated cells showed changes in morphology, saturation density, growth rate, and serum dependence. By the 5th passage they acquired the ability to grow in 0.3% agarose, and by the 30th passage they gave rise to fully transformed foci that grew in agarose, in agar, and in liquid suspension. This progression was rapidly accelerated if the cultures derived from 5-azacytidine-treated cells were exposed for 48 h to the carcinogen benzo[a]pyrene. Results of these studies provide evidence that aberrations in DNA methylation may be one of a series of critical events during the course of multistage carcinogenesis and thus enhance the evolution of tumor cells.