THE DROSOPHILA TOLL GENE FUNCTIONS ZYGOTICALLY AND IS NECESSARY FOR PROPER MOTONEURON AND MUSCLE DEVELOPMENT
THE DROSOPHILA TOLL GENE FUNCTIONS ZYGOTICALLY AND IS NECESSARY FOR PROPER MOTONEURON AND MUSCLE DEVELOPMENT
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DOI：
10.1006/dbio.1995.1134
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发表时间：
1995-05-01
影响因子：
2.7
通讯作者：
KESHISHIAN, H
中科院分区：
文献类型：
--
作者：
HALFON, MS;HASHIMOTO, C;KESHISHIAN, H
Toll基因是果蝇的一个母系必需基因，编码一种跨膜蛋白，在胚胎背腹图案形成中具有重要功能。Toll蛋白在合子中广泛表达，但其在晚期胚胎发生中的作用尚未详细描述。我们研究了Toll蛋白在胚胎晚期中枢神经系统和躯体肌肉组织中的表达。Toll在肌肉组织中以动态模式表达，最初在每个半节中的几个肌纤维中表达，随后表达变窄至单个肌纤维对。合子Toll突变体用于研究Toll表达丧失的发育后果。我们发现Toll基因的一个或两个拷贝的丢失导致运动神经元数量和肌肉模式的广泛缺陷。运动神经元的丧失阻止某些肌纤维接受它们的野生型神经支配。在突变体的失神经导致从附近的神经分支的侧支发芽，并导致异位放置的运动末梢的外观。有限的表现力表明，Toll只是适当的运动神经元和肌肉规格所需的几个基因之一。(C)出版社：Academic Press
Toll is a maternally required Drosophila gene that encodes a transmembrane protein with an important function in embryonic dorsal-ventral patterning. The Toll protein is widely expressed zygotically, but its roles in late embryogenesis have not been described in detail. We have examined the expression of Toll protein in the late embryonic central nervous system and somatic musculature. Toll is expressed in a dynamic pattern in the musculature, initially in several muscle fibers in each hemisegment, with a later narrowing of expression to a single muscle fiber pair. Zygotic Toll mutants were used to investigate the developmental consequences of loss of Toll expression. We found that loss of one or both copies of the Toll gene leads to widespread defects in motoneuron number and muscle patterning. Loss of motoneurons prevents certain muscle fibers from receiving their wild-type innervation. Denervation in the mutants results in collateral sprouting from nearby nerve branches and leads to the appearance of ectopically placed motor endings. The limited expressivity observed suggests that Toll is only one of several genes required for proper motoneuron and muscle specification. (C) 1995 Academic Press,Inc.