Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts
Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts
复制标题
葫芦异种嫁接苗接穗与砧木间长距离传递mRNA的鉴定
DOI：
10.3390/ijms21155253
复制
发表时间：
2020-07
影响因子：
5.6
通讯作者：
Zhang Wenna
中科院分区：
文献类型：
--
作者：
Liu Wenqian;Xiang Chenggang;Li Xiaojun;Wang Tao;Lu Xiaohong;Liu Zixi;Gao Lihong;Zhang Wenna
嫁接已被广泛用于改善作物生产中的植物生长和耐受性，以及用于阐明器官间通讯中从供体组织到受体组织的系统性mRNA信号传导。在这项研究中，我们研究了黄瓜（Csa）和南瓜（Cmo）的嫁接伴侣相互作用机制，使用大规模的内源mRNA运输。结果表明，大多数移动的转录本遵循从源到汇的分配路径。基因本体论（GO）富集和京都基因和基因组百科全书（KEGG）分析表明，mRNA的移动功能是普遍共同的和个别特定的。采用RT-PCR（逆转录PCR）、RT-qPCR（逆转录定量真实的时间PCR）和克隆测序鉴定异种移植物中远端组织之间的mRNA移动性，以估计78.75%的选定移动的转录本。结合生物信息学分析和RT-qPCR鉴定，推测黄瓜接穗-砧木-接穗之间存在Csa下移和Cmo上移的反馈信号环，Csa接穗下移的mRNA参与了碳固定和氨基酸合成途径，Cmo根系接收Csa下移的mRNA，然后将相应的Cmo上移的mRNA传递给接穗，从而提高黄瓜接穗的光合作用。这形成了一个接穗-砧木-接穗的反馈信号回路，解释了为什么南瓜砧木在工业上提高了黄瓜产量，这是用于器官通讯和介导异接葫芦科作物的光合作用过程。
Grafting has been widely used to improve plant growth and tolerance in crop production, as well as for clarifying systemic mRNA signaling from donor to recipient tissues in organ-to-organ communication. In this study, we investigated graft partner interaction mechanisms of Cucumis sativus (Csa) and Cucurbita moschata (Cmo) using a large-scale endogenous mRNA transport. The results indicated that most mobile transcripts followed an allocation pathway from source to sink. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that mRNA mobility functions are universally common and individually specific. Identification of mRNA mobility between distant tissues in heterografts with RT-PCR (reverse transcription PCR), RT-qPCR (reverse transcriptional quantitative real time PCR), and clone sequencing were used to estimate 78.75% of selected mobile transcripts. Integration of bioinformatic analysis and RT-qPCR identification allowed us to hypothesize a scion-to-rootstock-to-scion feedback signal loop of Csa move-down and Cmo move-up mRNAs, where Csa scion move-down mRNAs were involved in carbon fixation and biosynthesis of amino acid pathways, and Cmo root received Csa move-down mRNA and then delivered the corresponding Cmo upward mRNA to scion to improve photosynthesis of cucumber scion. This formed a feedback signal loop of scion-to-rootstock-to scion to explain why pumpkin rootstock enhanced cucumber production in the industry, which was utilized for organ communication and mediates photosynthesis processes in heterograft cucurbit crops.
登录
查看更多内容
影响因子：
18
作者：
Zhaoliang Zhang;Yi Zheng;B. Ham;Jieyu Chen;A. Yoshida;L. Kochian;Z. Fei;W. J. Lucas
通讯作者：
Zhaoliang Zhang;Yi Zheng;B. Ham;Jieyu Chen;A. Yoshida;L. Kochian;Z. Fei;W. J. Lucas
影响因子：
4.8
作者：
Gaion, Lucas Aparecido;Carvalho, Rogerio Falleiros
通讯作者：
Carvalho, Rogerio Falleiros
影响因子：
5.6
作者：
DREHER, TW;RAO, ALN;HALL, TC
通讯作者：
HALL, TC
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
影响因子：
7.2
作者：
Hu, Chaoyang;Ham, Byung-Kook;Lucas, William J.
通讯作者：
Lucas, William J.