Transcriptional profiling of foam cells in response to hypercholesterolemia.
Transcriptional profiling of foam cells in response to hypercholesterolemia.
复制标题
泡沫细胞响应高胆固醇血症的转录谱。
DOI：
10.1016/j.gdata.2016.06.006
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Paul,Antoni
中科院分区：
文献类型：
--
作者：
Goo,Young-Hwa;Yechoor,VijayK;Paul,Antoni
高胆固醇血症是动脉粥样硬化发展的主要危险因素。动脉巨噬细胞，或泡沫细胞，吸收和处理沉积在动脉中的脂蛋白颗粒，并将这些颗粒携带的大部分胆固醇储存在细胞质中。然而，暴露在不同胆固醇水平下对泡沫细胞的影响仍然知之甚少。鉴于巨噬细胞对环境变量的显著可塑性，理想情况下，巨噬细胞生物学研究应在其发挥其生理功能的环境中进行，即泡沫细胞中的动脉粥样硬化病变。我们使用了动脉粥样硬化的小鼠模型，即载脂蛋白E缺陷小鼠，研究了泡沫细胞对喂食西式饮食引起的血浆胆固醇短期和长期升高的转录反应。来自这项研究的微阵列数据集已经在NCBI的基因表达总览中登记，登录号为GSE70619。在这里，我们提供了关于实验装置、激光捕获显微切割分离RNA的详细信息，以及用于RNA扩增和通过微阵列和实时定量PCR进行分析的方法学。
Hypercholesterolemia is a main risk factor for atherosclerosis development. Arterial macrophages, or foam cells, take-up and process lipoprotein particles deposited in arteries, and store much of the cholesterol carried by these particles in their cytoplasm. However, the effects of exposure to different cholesterol levels on foam cells remain poorly understood. Given the remarkable plasticity of macrophages in response to environmental variables, studies on macrophage biology should ideally be performed in the environment where they exert their physiological functions, namely atherosclerotic lesions in the case of foam cells. We used a mouse model of atherosclerosis, the apolipoprotein E-deficient mouse, to studyin vivothe transcriptional response of foam cells to short- and long-term elevations in plasma cholesterol, induced by feeding mice a western type diet. The microarray data sets from this study have been deposited in NCBI's Gene Expression Omnibus under the accession number GSE70619. Here we provide detailed information on the experimental set-up, on the isolation of RNA by laser capture microdissection, and on the methodology used for RNA amplification and analysis by microarray and quantitative real-time PCR.